Human ADH/VP/AVP ELISA Kit — FAQ for Endocrinology, Nephrology, and Water Balance Researchers

Human ADH/VP/AVP ELISA Kit: Your Questions Answered

Antidiuretic hormone (ADH) , also known as vasopressin (VP) or arginine vasopressin (AVP) , is a 9-amino-acid cyclic nonapeptide synthesized in the hypothalamus and released from the posterior pituitary. Its name tells two stories: as ADH, it tells the kidney collecting duct to insert aquaporin-2 channels and reabsorb water, concentrating urine and preserving body fluid osmolality; as vasopressin, it constricts blood vessels and raises blood pressure. AVP is also a neurotransmitter in the brain, modulating social behavior, stress responses, and memory.

Clinically, AVP measurement is used for studies involving diabetes insipidus (central or nephrogenic), syndrome of inappropriate ADH secretion (SIADH) , ectopic AVP production, and psychogenic water intoxication. AVP is also implicated in heart failure, cirrhosis, and autism spectrum disorder.

Abbkine's Human Anti-diuretic hormone/vasopressin/arginine vasopressin (ADH/VP/AVP) ELISA Kit (KTE62629) provides researchers with a sensitive and specific tool for quantifying this critical neuropeptide in human samples. Here are answers to the most common questions about this kit and AVP detection.

What is the detection principle of this ADH/AVP ELISA kit?

This kit employs a competitive or sandwich ELISA format to quantitate AVP in samples. A specific antibody is pre-coated onto the microplate. When standards and samples are added, any AVP present binds to the immobilized antibody. After removing unbound substances, a detection reagent is added, followed by a substrate solution. Color development is inversely or directly proportional to the amount of AVP bound, and the intensity is measured at the appropriate wavelength.

What sample types can I use?

The kit is validated for serum, plasma, cell culture supernatants, and other biological fluids. This flexibility makes it suitable for both clinical research and in vitro neuropeptide release studies.

What is the sensitivity and detection range?

Abbkine's ELISA kits are engineered for high sensitivity and excellent specificity. For specific sensitivity information tailored to your expected concentration range, contact Abbkine's technical support team. The kit is designed to detect AVP in the low pg/mL range — essential because basal plasma AVP concentrations are often in the single-digit pg/mL range.

Does this kit cross-react with oxytocin or other neuropeptides?

No significant cross-reactivity or interference with AVP analogues has been observed. This specificity is critical given the structural homology between AVP and oxytocin (both are cyclic nonapeptides differing only in two amino acids).

How should I store and handle the kit?

• Unopened kit: Store at 2–8°C
• Opened kit: Follow protocol-specific storage instructions
• Shipping: Gel pack with blue ice

Critical handling tips:

• AVP is a short-lived peptide — process samples promptly
• Extraction protocols may be required for plasma samples to remove interfering binding proteins
• Avoid repeated freeze-thaw cycles
• Allow all reagents to warm to room temperature for at least 30 minutes before opening
• Run samples and standards in duplicate or triplicate

What applications is this kit suitable for?

This kit is designed for research use only — not for human or clinical diagnosis. Applications include:

• Diabetes insipidus research (central and nephrogenic)
• SIADH (syndrome of inappropriate ADH secretion) studies
• Heart failure and cirrhosis research (AVP in water retention)
• Autism spectrum disorder and social behavior research
• Stress and HPA axis studies
• Hyponatremia and water balance disorder research
• Ectopic AVP production in tumors

Why measure AVP?

AVP is a multifunctional neuropeptide with broad clinical and research relevance:

• It is the master regulator of water balance — controlling urine concentration and body fluid osmolality
• It is a vasoconstrictor — modulating blood pressure and vascular tone
• It is a neurotransmitter — influencing social cognition, stress, and memory
• AVP dysregulation is implicated in diabetes insipidus, SIADH, heart failure, and autism

What's included in the kit?

A complete, ready-to-use kit.

📌 Reference Link: Human Anti-diuretic hormone/vasopressin/arginine vasopressin (ADH/VP/AVP) ELISA Kit — KTE62629