Human MMP-13 ELISA Kit — Application Scenarios for Osteoarthritis, Bone Remodeling, and Cancer Research
MMP-13: The Collagenase That Chews Through Your Cartilage
Matrix metalloproteinase-13 (MMP-13) , also known as Collagenase 3 (UniProt: P45452, Gene ID: 4322, Chr 11q22.3), is a secreted zinc-dependent endopeptidase that preferentially cleaves type II fibrillar collagen — the structural backbone of articular cartilage — at the Gly⁷⁷⁵-Leu⁷⁷⁶ site. It also degrades aggrecan core protein, completing the one-two punch that dismantles the cartilage matrix in osteoarthritis. MMP-13 is the primary collagenase in osteoarthritic cartilage, and its expression correlates with disease severity and cartilage loss.
Beyond osteoarthritis, MMP-13 is involved in bone development, bone mineralization, wound healing, and tumor progression and metastasis. In murine surgical models of osteoarthritis, uninhibited MMP-13 catalytic activity serves as the primary driver of localized structural joint destruction.
Abbkine's Human Matrix Metalloproteinase 13 (MMP-13) ELISA Kit (KTE62620) provides a quantitative, sandwich ELISA solution for measuring this critical collagenase. Here's how researchers across different fields are putting it to work.
Osteoarthritis Research: The Primary Collagenase of Cartilage Destruction
MMP-13 is the dominant collagenase in osteoarthritic cartilage. Osteoarthritis researchers use this kit to:
- Quantify MMP-13 in synovial fluid, serum, and cartilage tissue lysates
- Monitor cartilage degradation dynamics in OA progression
- Evaluate the efficacy of MMP-13 inhibitors and DMOADs in preclinical models
- Correlate MMP-13 levels with radiographic joint space narrowing
- Investigate MMP-13 as a biomarker for early OA diagnosis and progression
The kit delivers quantitative data from serum, plasma, cell culture supernatants, and other biological fluids — enabling both preclinical and translational studies.
Bone Remodeling and Skeletal Biology
MMP-13 plays critical roles in bone development, mineralization, and ossification. Skeletal biologists use this kit to:
- Quantify MMP-13 in bone tissue lysates and osteoblast/osteoclast cultures
- Study endochondral ossification and growth plate dynamics
- Investigate MMP-13's role in bone fracture healing
- Monitor MMP-13 during osteogenic differentiation
Cancer Research: Tumor Invasion and Metastasis
MMP-13 is upregulated in multiple cancers and promotes ECM degradation, tumor invasion, and metastasis. Oncology researchers use this kit to:
- Quantify MMP-13 in tumor tissue lysates and patient serum/plasma
- Study MMP-13's role in tumor microenvironment remodeling
- Investigate MMP-13 as a prognostic biomarker in breast, lung, and colorectal cancers
- Monitor MMP-13 levels in response to anti-metastatic therapies
Why Choose This Kit for Your MMP-13 Research?
| Feature | Benefit |
|---|---|
| Two-site sandwich ELISA | Quantitative, specific detection |
| Human reactivity | Directly applicable to human samples |
| High specificity | No significant cross-reactivity with MMP-1, MMP-8, or other collagenases |
| Multiple sample types | Serum, plasma, synovial fluid, cell culture supernatants, tissue lysates |
| Complete components | Ready-to-use — pre-coated plate, standards, detection antibodies, all buffers |
Kit Components at a Glance
- Human MMP-13 microplate (pre-coated with capture antibody)
- Human MMP-13 standard
- Human MMP-13 detection antibody
- HRP-conjugate or Streptavidin-HRP
- Standard diluent, assay buffer, HRP substrate, stop solution, wash buffer, plate covers
🔬 Which research field does your work belong to?
Osteoarthritis Research | Bone Remodeling | Cancer Metastasis | Wound Healing | Other
Share your application with us — we'd love to hear how you're using this kit in your studies!
📌 Reference Link: Human Matrix Metalloproteinase 13 (MMP-13) ELISA Kit — KTE62620
