KTA1030 | LDH Cytotoxicity Assay Kit — The 30-Minute Colorimetric Assay That Finally Makes Cytotoxicity Testing Simple, Reliable, and Reproducible

The Core Pain Point of Conventional Cytotoxicity Detection

Every lab focusing on anticancer drugs, immunotherapy or organoid models relies on LDH release to quantify cell damage, yet standard LDH detection schemes bring persistent troubles. Traditional detection consumes precious limited supernatant, generates unstable absorbance signals, and demands repeated pre-experiments to adjust cell seeding density. When working with rare patient-derived primary cells or tiny 3D spheroid cultures, wasting sample volume on unoptimized assays severely slows down compound screening pipelines and raises overall lab R&D costs.

Detection Mechanism of Abbkine KTA1030

This colorimetric kit quantifies cytotoxicity by measuring lactate dehydrogenase leaked into culture media after cell membrane rupture. The complete reaction system relies on NADH-coupled enzymatic cascade reactions: LDH catalyzes lactate conversion to pyruvate while reducing NAD⁺ to NADH, which further drives MTT reduction to form stable chromogenic products with maximum absorbance at 565 nm. The color intensity maintains a strict linear correlation with the number of lysed target cells, supporting accurate quantitative calculation of cell death rates without radioactive labeling or repeated cell lysis operations.

Full Kit Components & Standardized Operating Protocol

Abbkine KTA1030 comes with all matched reagents required for one-step detection: Assay Buffer, Lactic Acid Solution, MTT Solution, PES Solution, LDH Positive Control, NAD+ Solution and 10% Triton X-100 lysis reagent. The standardized workflow is straightforward for all researchers: transfer culture supernatant to a new 96-well plate, mix thoroughly with reaction liquid, incubate for 30 minutes at room temperature, then read OD values at 565 nm via microplate readers. For 3D spheroid samples, collected medium only needs a 1:10 dilution with assay buffer to eliminate matrix interference.

Wide Sample Compatibility & Key Functional Advantages

KTA1030 fits nearly all mainstream cell research models, including adherent tumor cells, suspension primary NK/T cells, 3D tumor organoids and microbial biofilm cultures. It supports accurate detection for 10,000 to 1,000,000 cells per well, covering basic cytotoxicity tests, CAR-T safety evaluation, anticancer drug screening and bioink biocompatibility verification. All reagents are pre-formulated to minimize manual errors, and batch-to-batch consistency eliminates frequent cell density optimization for different sample types. Up to 7 peer-reviewed publications have validated its stable detection performance.

Storage, Shipping & Critical Safety Precautions

All kit components must be stored at -20°C away from light after receipt, with a total shelf life of 6 months. The product is shipped with blue ice gel packs to guarantee enzyme activity during cross-regional transportation. A vital reminder: KTA1030 is strictly for laboratory basic research only, and cannot be used for human clinical diagnosis. Users shall bear full responsibility for any patent infringement risks caused by unauthorized commercial application of this detection system.
Product Link: https://www.abbkine.com/product/ldh-cell-proliferation-and-cytotoxicity-assay-kit-kta1030/