CheKine™ Micro Glucose-6-Phosphatase (G6P) Activity Assay Kit — FAQ for Glucose Metabolism Researchers
CheKine™ Micro Glucose-6-Phosphatase (G6P) Activity Assay Kit: Your Questions Answered
Glucose-6-phosphatase (G6Pase, EC 3.1.3.9) is widely present in animals, plants, microorganisms, and cells[reference:63]. It is the rate-limiting enzyme in the hydrolysis of glucose-6-phosphate by gluconeogenesis, playing an important role in ensuring blood glucose homeostasis[reference:64]. G6Pase deficiency leads to glycogen storage disease type I (von Gierke disease).
Abbkine's CheKine™ Micro Glucose-6-Phosphatase (G6P) Activity Assay Kit (KTB1014) provides a simple method for detecting G6Pase activity[reference:65]. Here are answers to the most common questions about this kit.
What is the detection principle?
G6Pase catalyzes the hydrolysis of glucose-6-phosphate to glucose and inorganic phosphate. The kit measures G6Pase activity by quantifying the amount of inorganic phosphate (Pi) released from glucose-6-phosphate. The released phosphate reacts with a chromogenic reagent to produce a colored product, measured spectrophotometrically. The increase in absorbance is proportional to G6Pase activity.
What sample types can I use?
The kit is suitable for detecting G6Pase activity in:
- Animal tissues (liver, kidney, intestine)
- Cultured cells (hepatocytes, cell lines)
- Plant tissues
- Microorganisms[reference:66]
What are the key features?
| Feature | Benefit |
|---|---|
| Simple method | Easy-to-follow protocol[reference:67] |
| Detailed instructions | Sample preparation and calculation methods provided[reference:68] |
| Simple operation steps | Streamlined workflow[reference:69] |
| 12-month stability | Store at 4°C[reference:70] |
How should I store and handle the kit?
- Storage: Store at 4°C and protect from light upon receipt[reference:71]
- Shelf life: 12 months from receipt[reference:72]
- Shipping: Gel pack with blue ice[reference:73]
Critical handling tips:
- Prepare samples freshly for best results
- Follow the sample preparation protocol carefully
- Run samples and standards in duplicate or triplicate
What applications is this kit suitable for?
This kit is designed for research use only[reference:74]. Applications include:
- Glucose metabolism research (gluconeogenesis, glycogenolysis)
- Diabetes and metabolic disease studies
- Liver function research (hepatic glucose production)
- Nutritional studies (dietary effects on glucose metabolism)
- Drug development (anti-diabetic compounds targeting G6Pase)
- Plant metabolism research
Why measure G6Pase activity?
G6Pase is a critical enzyme in glucose homeostasis:
- It catalyzes the final step of gluconeogenesis and glycogenolysis
- It releases glucose into the bloodstream from the liver and kidney
- Dysregulation is associated with type 2 diabetes and metabolic syndrome
- Inhibitors of G6Pase are potential anti-diabetic therapeutics
Has this kit been validated in publications?
Yes — this kit has been cited in 1 publication[reference:75], providing real-world validation.
What's included in the kit?
| Component | Included |
|---|---|
| Extraction Buffer | ✅ |
| Reagent I | ✅ |
| Reagent II | ✅ |
A complete, ready-to-use kit.
📌 Reference Link: CheKine™ Micro Glucose-6-Phosphatase (G6P) Activity Assay Kit — KTB1014
