EliKine™ Human IL-10 ELISA Kit — Application Scenarios for Immunology and Inflammation Research

IL-10: The Master Anti-Inflammatory Cytokine

Interleukin 10 (IL-10) is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes[reference:119]. This cytokine has pleiotropic effects in immunoregulation and inflammation[reference:120]. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages[reference:121]. It also enhances B cell survival, proliferation, and antibody production[reference:122]. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway[reference:123].

Abbkine's EliKine™ Human IL-10 ELISA Kit (KTE6019) provides researchers with a sensitive, specific, and reliable tool for quantifying this essential anti-inflammatory cytokine in human samples. With 3 publications citing this kit[reference:124], it's a trusted tool in the research community. Here's how researchers across different fields are putting it to work.

Immunology: Immune Suppression and Tolerance

IL-10 is the master anti-inflammatory cytokine that maintains immune homeostasis. Immunologists use this kit to:

• Quantify IL-10 secretion in regulatory T cell (Treg) assays
• Monitor immune tolerance and suppression in autoimmune disease models
• Assess the effects of immunosuppressive compounds on IL-10 production
• Study the role of IL-10 in allergy and hypersensitivity

The sandwich ELISA format delivers quantitative data from serum, plasma, or cell culture supernatants[reference:125].

Autoimmune Disease Research: Restoring Immune Balance

IL-10 plays a critical role in preventing autoimmunity by suppressing inflammatory responses. Autoimmune disease researchers leverage this kit to:

• Monitor IL-10 levels in autoimmune disease models (rheumatoid arthritis, multiple sclerosis, lupus)
• Study IL-10 as a therapeutic target for autoimmune disorders
• Assess the effects of immunomodulatory therapies on IL-10 production
• Investigate IL-10 dysregulation in autoimmune pathogenesis

High sensitivity (1 pg/mL LOD) ensures detection of even low-level IL-10 in samples[reference:126].

Infectious Disease Research: Host Defense and Immune Evasion

IL-10 plays a dual role in infection — limiting immunopathology while also potentially contributing to pathogen persistence. Infectious disease researchers use this kit to:

• Monitor IL-10 levels in viral and bacterial infection models
• Study IL-10's role in limiting inflammatory damage during infection
• Investigate pathogen-mediated IL-10 induction as an immune evasion strategy
• Assess IL-10 as a biomarker of infection severity

Cancer Research: Tumor Immune Evasion

IL-10 can be produced by tumor cells and immune cells in the tumor microenvironment to suppress anti-tumor immunity. Oncology researchers use this kit to:

• Quantify IL-10 in tumor microenvironment studies
• Monitor IL-10 levels in cancer patient serum/plasma
• Study IL-10's role in immune evasion and tumor progression
• Assess the effects of immunotherapy on IL-10 production

Why Choose This Kit for Your IL-10 Research?

Kit Components at a Glance

• Human IL-10 microplate
• Human IL-10 standard
• Human IL-10 detect antibody
• EliKine™ Streptavidin-HRP
• Standard diluent, assay buffer, HRP substrate, stop solution, wash buffer, plate covers[reference:129]

🔬 Which research field does your work belong to?
Immunology | Autoimmune Disease | Infectious Disease | Cancer Research | Other
Share your application with us — we'd love to hear how you're using this kit in your studies!

📌 Reference Link: EliKine™ Human IL-10 ELISA Kit — KTE6019