EliKine™ Human CD54 ELISA Kit (KTE6003)

EliKine™ Human CD54 (sICAM-1) ELISA Kit: Is It Right for Your Lab?

CD54, also known as ICAM-1 (Intercellular Adhesion Molecule 1) , is a transmembrane protein of the immunoglobulin superfamily. It plays a central role in immune cell adhesion, leukocyte extravasation, and inflammatory responses. Soluble ICAM-1 (sICAM-1/CD54) is a well-established biomarker for inflammation, cardiovascular disease, and autoimmune disorders.

The EliKine™ Human CD54 (sICAM-1) ELISA Kit (KTE6003) from Abbkine offers a sensitive, specific, and complete solution for quantifying this important adhesion molecule. Here's our detailed review.

What's in the Box?

A complete, ready-to-use kit.

Advantages

1. Exceptional Sensitivity

This kit delivers an industry-leading limit of detection of just 7.8 pg/mL with a calibration range of 7.8–500 pg/mL. For researchers working with low-abundance sICAM-1 samples, this sensitivity is a game-changer.

2. Outstanding Specificity

No significant cross-reactivity or interference between human sICAM-1 and analogues. Your data reflects true sICAM-1 levels—not background noise from related adhesion molecules.

3. Broad Sample Compatibility

Validated for serum, plasma, cell culture supernatants, and other biological fluids—adaptable to most experimental designs.

4. Published Track Record

This kit has been cited in 1 publication—real-world validation from the research community.

5. Direct HRP Detection System

The HRP-conjugated detection antibody format eliminates the need for secondary antibody steps—simplifying the workflow and reducing assay time.

6. Complete Kit

All necessary components—microplate, standards, diluent, assay buffer, HRP-conjugate, substrate, stop solution, wash buffer, and plate covers—are included.

7. Reliable Storage and Shipping

• Unopened kit: store at 2–8°C
• Shipped with gel pack and blue ice

Disadvantages

1. Narrower Calibration Range

The 7.8–500 pg/mL range may require dilution for samples with very high sICAM-1 concentrations.

2. Human-Specific

This kit is specific to human CD54/sICAM-1—not validated for mouse, rat, or other species.

3. Research Use Only

Not approved for human or clinical diagnosis.

4. Assay Duration

Standard sandwich ELISA with a working time of 3–5 hours.

Who Should Buy This Kit?

✅ Buy this kit if you:

• Study inflammation, immunology, or cardiovascular disease
• Need to quantify human sICAM-1/CD54 in serum, plasma, or cell culture
• Require ultra-high sensitivity (7.8 pg/mL LOD)
• Value specificity—no cross-reactivity with related molecules
• Prefer a complete, ready-to-use kit
• Want a kit with published validation (1 citation)
• Appreciate the simplicity of direct HRP detection

❌ Consider alternatives if you:

• Need to detect mouse or rat ICAM-1
• Are working with samples containing very high sICAM-1 levels (>500 pg/mL)
• Require a faster assay (<3 hours)

Quick Specs Summary

📌 Reference Link: EliKine™ Human CD54 ELISA Kit — KTE6003