Dylight 488 Goat Anti-Rabbit IgG: Precision Green Fluorescence for Rabbit Primary Antibody Detection
The Gold Standard for Rabbit Detection
Rabbit primary antibodies are among the most widely used reagents in biomedical research—from neurobiology to cancer immunology. The secondary antibody you choose to detect them determines whether your data is publication-ready or plagued by high background and weak signal.
Abbkine's Dylight 488 Goat Anti-Rabbit IgG (A23220) is an affinity-purified polyclonal antibody targeting rabbit IgG whole molecules, with >95% purity confirmed by SDS-PAGE. Validated for FCM, ICC, and IF, with a recommended starting dilution of 1:50–1:1000. With 157 citations in peer-reviewed literature, A23220 is one of the most heavily validated green-channel anti-rabbit secondaries available.
Brightness That Sets the Standard
DyLight 488-antibody conjugates are brighter than Cy2 and FITC conjugates and match the brightness of Alexa Fluor 488 conjugates. This brightness advantage translates to lower working concentrations, reduced background, and cleaner images.
Photostability That Survives Long Exposures
DyLight 488 conjugates exhibit superior photostability—they fade less than FITC and Cy2 in mounting media, a property inherent to the DyLight 488 molecule itself. For researchers performing multi-channel imaging or extended acquisition sessions, this photostability is non-negotiable.
pH-Independent Fluorescence (pH 4–9)
DyLight fluorescent dyes maintain their fluorescence across a broad pH range (4 to 9) , making them ideal for samples subjected to antigen retrieval, fixation, or mounting media with variable pH.
Specificity: Heavy and Light Chain Recognition
A23220 reacts with whole molecule rabbit IgG, including both heavy and light chains, while showing no reactivity on non-immunoglobulin serum proteins.
Specifications at a Glance
| Parameter | A23220 |
|---|---|
| Immunogen | Rabbit IgG whole molecule |
| Host | Goat |
| Conjugate | Dylight 488 |
| Purity | >95% (SDS-PAGE) |
| Applications | FCM, ICC, IF |
| Dilution Range | 1:50–1:1000 |
| Citations | 157+ |
Why Choose A23220?
- Brightness: > FITC, > Cy2, = Alexa Fluor 488
- Photostability: Superior to FITC and Cy2
- pH Range: 4–9 (stable fluorescence)
- Purity: >95% by SDS-PAGE
📎 Reference: Dylight 488, Goat Anti-Rabbit IgG (A23220)
