Dylight 488, Goat Anti-Rabbit IgG

Product Information

  • Product name

    Dylight 488, Goat Anti-Rabbit IgG

  • Immunogen

    Rabbit IgG whole molecule

  • Host

    Goat

  • Reactivity

    Rabbit

  • Applications

    FCM, ICC, IF

  • Application notes

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:1000 dilutions for most fluorescent applications.

  • Clonality

    Polyclonal

  • Isotype

    Goat IgG

  • Purification

    Affinity purified using solid phase Rabbit IgG (H&L) with finally > 95% purity based on SDS-PAGE

  • Conjugate

    Dylight 488

Fig.1. DyLight fluorescent dyes are a new family of dyes with improved brightness. DyLight 488-antibody conjugates are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates. DyLight 549-antibody conjugates shows brighter influoresence than TRITC conjugates. Also, DyLight 594-antibody conjugates are noticeably brighter than Alexa 594 conjugates, and much brighter and more water soluble than Texas Red conjugates.

Fig.2. DyLight fluorescent dyes show improved photostability as well. DyLight 488 conjugates fade less than FITC and Cy2 conjugates in mounting media indicating that the DyLight 488 molecule is inherently more photostable in epifluorescence microscopes. DyLight 549 conjugates are about as photostable as Alexa 555 conjugates and slightly more photostable than Cy3 conjugates.

Fig.3. To use the DyLight Fluors with fluorescent imagers, use a spectral line of the blue laser diode for DyLight 405, a cyan (488 nm) laser for DyLight 488, a green (526 nm) laser for DyLight 550 and 594, and a red (633 nm) laser for DyLight 633 and 650. The DyLight 680, 755 and 800 fluors are compatible with laser- and filter-based infrared imaging instruments that emit in the 700 nm, 750 nm and 800 nm region of the spectrum, respectively.

Product Properties

  • Formulation

    Liquid solution

  • Features & Benefits

    DyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).

  • Storage buffer

    Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative, 1% BSA as stablizer and 50% Glycerol.

  • Storage instructions

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.

  • Others

    The antibody reacts with whole molecule rabbit IgG. It also reacts with heavy chains of rabbit IgG, and light chains of all other rabbit immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.

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Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

  1. Allicin attenuates calcium oxalate crystal deposition in the rat kidney by regulating gap junction function

    Yongchang Lai, Xiongfa Liang, Fangling Zhong, et al. J Cell Physiol. 2019 Jun;234(6):9640-9651.

  2. Melatonin Enhances Proliferation and Modulates Differentiation of Neural Stem Cells Via Autophagy in Hyperglycemia

    HAOYUAN LI, YANMIN ZHANG, SHANGMING LIU, et al. Stem Cells. 2019 Apr;37(4):504-515.

  3. FCPR16, a novel phosphodiesterase 4 inhibitor, produces an antidepressant-like effect in mice exposed to chronic unpredictable mild stress

    Qiuping Zhong, Hui Yu, Chang Huang, et al. Prog Neuropsychopharmacol Biol Psychiatry. 2019 Mar 2;90:62-75.

  4. Acute exposure to triphenyl phosphate (TPhP) disturbs ocular development and muscular organization in zebrafish larvae

    Qipeng Shi, Mirabelle M.P.Tsui, ChenyanHu, et al. Ecotoxicol Environ Saf. 2019 Apr 25;179:119-126.

  5. FLOT1 promotes tumor development, induces epithelial–mesenchymal transition, and modulates the cell cycle by regulating the Erk/Akt signaling pathway in lung adenocarcinoma

    Louqian Zhang, Yuan Mao, Qixing Mao, et al. Thorac Cancer. 2019 Apr;10(4):909-917.

  6. The ΦCPG1 chlamydiaphage can infect Chlamydia trachomatis and significantly reduce its infectivity

    Shijuan Wei, Quanzhong Liu, Tingting Lian and Lili Shao. Virus Res. 2019 Apr 25;267:1-8.

  7. CDK11p110 plays a critical role in the tumorigenicity of esophageal squamous cell carcinoma cells and is a potential drug target

    Yue Du, Dan Yan, Yongliang Yuan, et al. Cell Cycle. 2019 Feb;18(4):452-466.

  8. Direct and indirect coculture of mouse hepatic progenitor cells with mouse embryonic fibroblasts for the generation of hepatocytes and cholangiocytes

    Tao Zhou, Wei Wang, Yasen Aimaiti, et al. Cytotechnology. 2019 Feb;71(1):267-275.

  9. Zinc-finger protein YY1 suppresses tumor growth of human nasopharyngeal carcinoma by inactivating c-Myc–mediated microRNA-141 transcription

    Mengna Li, Yukun Liu, Yanmei Wei, et al. J Biol Chem. 2019 Apr 12;294(15):6172-6187.

  10. Dimethylated histone H3 lysine 9 is dispensable for the interaction between developmentalpluripotency-associated protein 3 (Dppa3) and ten-eleven translocation 3 (Tet3) in somatic cells.

    Qian-Qian Wang, Yu-Mei Zhang, Xia Zhong, et al. Reprod Fertil Dev. 2018 Aug 13

  11. Requirement of Cellular Protein CCT7 for the Replication of Fowl Adenovirus Serotype 4 (FAdV-4) in Leghorn Male Hepatocellular Cells Via Interaction with the Viral Hexon Protein

    Junfeng Gao, Mingliang Zhao, Xueyan Duan, et al. Viruses. 2019 Jan 27;11(2).

  12. Alteration of the retinoid acid-CBP signaling pathway in neural crest induction contributes to enteric nervous system disorder

    Cheng Li, Rong Hu, Nali Hou, et al. Front Pediatr. 2018 Dec 3;6:382

  13. Ski‐related novel protein suppresses the development of diabetic nephropathy by modulating transforming growth factor‐β signaling and microRNA‐21 expression

    Yuanyuan Wang, Lingling Liu, Wei Peng, et al. 

  14. Hyperglycemia Altered the Fate of Cardiac Stem Cells to Adipogenesis through Inhibiting the β-Catenin/TCF-4 Pathway

    Zhang X, Meng K, et al. Cellular Physiology and Biochemistry. 2018; 49(6): 2254-2263.

  15. Stimulation of mouse vibrissal follicle growth by recombinant human fibroblast growth factor 20

    Xue-Gang, XuLin Gong, et al. Biotechnology Letters. 2018; 40(6): 1009-1014.

  16. Invasion of midgut epithelial cells by a persistently transmitted virus is mediated by sugartransporter 6 in its insect vector.

    Faliang Qin, Wenwen Liu, et al. PLoS Pathogens. 2018; 14(7): e1007201.

  17. TGFβ signaling controls intrahepatic bile duct development may through regulating the Jagged1‐Notch‐Sox9 signaling axis

    Wei Wang, Yuan Feng, et al. Journal of Cellular Physiology. 2018; 233(8): 5780-5791.

  18. Bisphenol A induced male germ cell apoptosis via IFNβ-XAF1-XIAP pathway in adult mice

    XiaoJiang, Li Yin, et al. Toxicology and Applied Pharmacology. 2018; 355: 247-256.

  19. Knockdown of Bone Morphogenetic Proteins Type 1a Receptor (BMPR1a) in Breast Cancer Cells Protects Bone from Breast Cancer-Induced Osteolysis by Suppressing RANKL Expression

    Liu Y, Yuan W, et al. Cell Physiology and Biochemmistry. 2018; 45(5): 1759-1771.

  20. SNAI1, an endothelial–mesenchymal transition transcription factor, promotes the early phase of ocular neovascularization

    Jia-Xing Sun, Tian-Fang Chang, et al. Angiogenesis. 2018; 21(3): 635-652.

  21. Inorganic Self-Assembled Bioactive Artificial Proto-Osteocells Inducing Bone Regeneration

    Shi M, Yang R, Li Q, et al. ACS Appl Mater Interfaces. 2018, 10(13): 10718-10728.

  22. A "Driver Switchover" Mechanism of Influenza Virus Transport from Microfilaments to Microtubules

    Zhang LJ, Xia L, Liu SL, et al. ACS Nano. 2018, 12(1): 474-484.

  23. Quantitative Proteomics Reveals the Regulatory Networks of Circular RNA CDR1as in Hepatocellular Carcinoma Cells

    Yang X, Xiong Q, Wu Y, et al. J Proteome Res. 2017 6;16(10): 3891-3902.

  24. Neuroglobin boosts axon regeneration during ischemic reperfusion via p38 binding and activation depending on oxygen signal

    Xiong XX, Pan F, Chen RQ, et al. Cell Death Dis, 2018, 9(2): 163.

  25. Silencing PPA2 inhibits human epithelial ovarian cancer metastasis by suppressing the Wnt/β-catenin signaling pathway

    Niu H, Zhou W, Xu Y, et al. Oncotarget, 2017, 8(44): 76266-76279.

  26. PD-L1 confers glioblastoma multiforme malignancy via Ras binding and Ras/Erk/EMT activation

    Qiu XY, Hu DX, Chen WQ, et al. Biochim Biophys Acta, 2018, 1864(5 Pt A): 1754-1769.

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    Shang J, Xia T, Han QQ, et al. Proteomics, 2018, 18(2).

  28. Probiotic Mixture Golden Bifido Prevents Neonatal Escherichia coli K1 Translocation via Enhancing Intestinal Defense

    Zeng Q, He X, Puthiyakunnon S, et al. Front Microbiol, 2017, 8: 1798.

  29. Perfluorohexadecanoic acid increases paracellular permeability in endothelial cells through the activation of plasma kallikrein-kinin system

    Liu QS, Hao F, Sun Z, et al. Chemosphere, 2018, 190: 191-200.

  30. Peli1 Contributions in Microglial Activation, Neuroinflammatory Responses and Neurological Deficits Following Experimental Subarachnoid Hemorrhage

    Huang XP, Peng JH, Pang JW, et al. Front Mol Neurosci, 2017, 10: 398.

  31. Biphasic activation of nuclear factor-κB and expression of p65 and c-Rel following traumatic neuronal injury

    Zhang H, Zhang D, Li H, et al. Int J Mol Med, 2018, 41(6): 3203-3210.

  32. IL‐6 increases podocyte motility via MLC‐mediated focal adhesion impairment and cytoskeleton disassembly

    He F F, Bao D, Su H, et al. J Cell Physiol, 2018.

  33. Curc-mPEG454, a PEGylated Curcumin Derivative, Improves Anti-inflammatory and Antioxidant Activities: a Comparative Study

    Cheng F, Chen Y, Zhan Z, et al. Inflammation, 2018, 41(2): 579-594.

  34. Altered expression of mTOR and autophagy in term normal human placentas

    Zhang Q X, Na Q and Song W Rom J Morphol Embryol, 2017; 58(2): 517-526.

  35. Neuroprotective Effect of β-Caryophyllene on Cerebral Ischemia-Reperfusion Injury via Regulation of Necroptotic Neuronal Death and Inflammation: In Vivo and in Vitro

    Yang M, Lv Y, Tian X, et al. Front Neurosci, 2017, 26; 11: 583.

  36. Trps1 is associated with the multidrug resistance of lung cancer cell by regulating MGMT gene expression

    Liu H, Liao Y, Tang M, et al. Cancer Med, 2018.

  37. Endothelial cell SHP-2 negatively regulates neutrophil adhesion and promotes transmigration by enhancing ICAM-1-VE-cadherin interaction

    Yan M, Zhang X, Chen A, et al. FASEB J, 31(11): 4759-4769.

  38. Digital Single Virus Electrochemical Enzyme-Linked Immunoassay for Ultrasensitive H7N9 Avian Influenza Virus Counting

    Wu Z, Guo WJ, Bai YY, et al. Anal Chem, 2018, 90(3): 1683-1690.

  39. SGK1 inhibition induces autophagy-dependent apoptosis via the mTOR-Foxo3a pathway

    Liu W, Wang X, Liu Z, et al. Br J Cancer, 10; 117(8): 1139-1153.

  40. Integrated Proteomic and Transcriptomic Analysis Reveals Long Noncoding RNA HOX Transcript Antisense Intergenic RNA (HOTAIR) Promotes Hepatocellular Carcinoma Cell Proliferation by Regulating Opioid Growth Factor Receptor (OGFr)

    Wu Y, Xiong Q, Li S T, et al. MOLECULAR & CELLULAR PROTEOMICS, 2018, 17: 146-159.

  41. Decidual Macrophage Functional Polarization during abnormal Pregnancy due to Toxoplasma gondii: role for lilrB4

    Li Z D, Zhao M D, Li T, et al. Frontiers in Immunology, 2017.

  42. The Role of Pannexin3-Modified Human Dental Pulp-Derived Mesenchymal Stromal Cells in Repairing Rat Cranial Critical-Sized Bone Defects

    Song F, Sun H, Huang L, et al. Cellular Physiology and Biochemistry, 2017, 44: 2174–2188.

  43. Electroacupuncture at ST36 Increases Bone Marrow-Derived Interstitial Cells of Cajal via the SDF-1/CXCR4 and mSCF/Kit-ETV1 Pathways in the Stomach of Diabetic Mice

    Zhao J, An J and Liu S Evidence-Based Complementary and Alternative Medicine, 2018.

  44. Bisphenol A and estrogen induce proliferation of human thyroid tumor cells via an estrogen-receptor-dependent pathway

    Zhang T H, Wei F, Zhang J, et al. Archives of Biochemistry and Biophysics, 2017, 633: 29-39.

  45. Apolipoprotein E-Mimetic Peptide COG1410 Promotes Autophagy by Phosphorylating GSK-3 in Early Brain Injury Following Experimental Subarachnoid Hemorrhage

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  46. Coaxial 3D bioprinting of self-assembled multicellular heterogeneous tumor fibers

    Dai, Xingliang, et al. Scientific Reports 7 (2017).

  47. Dihydromyricetin modulates p62 and autophagy crosstalk with the Keap-1/Nrf2 pathway to alleviate ethanol-induced hepatic injury

    Qiu, Ping, et al. Toxicology Letters 274 (2017): 31-41.

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    Liang, Ying, et al. Oncology Letters 14.2 (2017): 2254-2260.

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    Zhou, Xiangxiang, et al. Oncology Reports (2017).

  50. Ten-Eleven Translocation-2 (Tet2) Is Involved in Myogenic Differentiation of Skeletal Myoblast Cells in Vitro

    Zhong, Xia, et al. Scientific Reports 7 (2017): 43539.

  51. Evaluation of PFOS-mediated neurotoxicity in rat primary neurons and astrocytes cultured separately or in co-culture

    Li, Zhenwei, et al. Toxicology in Vitro 38 (2017): 77-90.

  52. The mechanically activated p38/MMP-2 signaling pathway promotes bone marrow mesenchymal stem cell migration in rats

    Yang, Zihui, et al. Archives of Oral Biology 76 (2017): 55-60.

  53. Role of Wnt/β-catenin signaling pathway in the repair of intestinal mucosa associated with crypt stem cell in a rat model of abdominal compartment syndrome

    Lu T, Luo H, Liu J, et al. Int J Clin Exp Pathol, 2017, 10(2): 2351-2362

  54. Application of a microfluidic-based perivascular tumor model for testing drug sensitivity in head and neck cancers and toxicity in endothelium

    Jin, Dong, et al. Rsc Advances 6.35(2016):29598-29607.

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    Fu, Qing-Qing, et al. Cellular and Molecular Neurobiology (2016): 1-12.

  56. Oocyte Vitellogenesis Triggers the Entry of Yeast-Like Symbionts Into the Oocyte of Brown Planthopper (Hemiptera: Delphacidae)

    Nan, Guo-Hui, et al. Annals of the Entomological Society of America 109.5 (2016): 753-758.

  57. Inhibition of Blood-Brain Barrier Disruption by an Apolipoprotein E-Mimetic Peptide Ameliorates Early Brain Injury in Experimental Subarachnoid Hemorrhage

    Pang J, Chen Y, Kuai L, et al. Translational Stroke Research, 2016: 1-16

  58. Quantitative Proteomics Analysis Reveals Novel Insights into Mechanisms of Action of Long Noncoding RNA Hox Transcript Antisense Intergenic RNA (HOTAIR) in HeLa Cells

    Zheng, Peng, et al. Molecular & Cellular Proteomics 14.6 (2015): 1447-1463.

  59. Ricin-B-lectin enhances microsporidia Nosema bombycis infection in BmN cells from silkworm Bombyx mori.

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    Tian X, Peng J, Zhong J, et al. Journal of Neurochemistry, 2016.

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  65. Blocking mPTP on Neural Stem Cells and Activating the Nicotinic Acetylcholine Receptor α7 Subunit on Microglia Attenuate Aβ-Induced Neurotoxicity on Neural Stem Cells.

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  67. Diosgenin and 5-Methoxypsoralen Ameliorate Insulin Resistance through ER-α/PI3K/Akt-Signaling Pathways in HepG2 Cells.

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  68. Lipid reduces GLP-1 production via inhibiting NF-kappa B p65 and IL-6 in intestinal L cells and pancreatic alpha cells.

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Cat #: A23220

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  • Price
  • 100μl $40
  • 500μl $160

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