- Product name
Dylight 488, Goat Anti-Rabbit IgG
Rabbit IgG whole molecule
FCM, ICC, IF
- Application notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:1000 dilutions for most fluorescent applications.
Affinity purified using solid phase Rabbit IgG (H&L) with finally > 95% purity based on SDS-PAGE
Fig.1. DyLight fluorescent dyes are a new family of dyes with improved brightness. DyLight 488-antibody conjugates are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates. DyLight 549-antibody conjugates shows brighter influoresence than TRITC conjugates. Also, DyLight 594-antibody conjugates are noticeably brighter than Alexa 594 conjugates, and much brighter and more water soluble than Texas Red conjugates.
Fig.2. DyLight fluorescent dyes show improved photostability as well. DyLight 488 conjugates fade less than FITC and Cy2 conjugates in mounting media indicating that the DyLight 488 molecule is inherently more photostable in epifluorescence microscopes. DyLight 549 conjugates are about as photostable as Alexa 555 conjugates and slightly more photostable than Cy3 conjugates.
Fig.3. To use the DyLight Fluors with fluorescent imagers, use a spectral line of the blue laser diode for DyLight 405, a cyan (488 nm) laser for DyLight 488, a green (526 nm) laser for DyLight 550 and 594, and a red (633 nm) laser for DyLight 633 and 650. The DyLight 680, 755 and 800 fluors are compatible with laser- and filter-based infrared imaging instruments that emit in the 700 nm, 750 nm and 800 nm region of the spectrum, respectively.
- Features & Benefits
DyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).
- Storage buffer
Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative, 1% BSA as stablizer and 50% Glycerol.
- Storage instructions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Gel pack with blue ice.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.
The antibody reacts with whole molecule rabbit IgG. It also reacts with heavy chains of rabbit IgG, and light chains of all other rabbit immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.
Most popular with customers
Application: ELISA, IHC, WB
Application: ELISA, IHC, WB
Application: FCM, ICC, IF
Application: FCM, ICC, IF
Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.
Preparation methods for Biochemical
Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.
Antibody application protocols
Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.
- ♦ Antibody Western Blotting (WB) protocol
- ♦ Antibody Immunohistochemistry (IHC) protocol
- ♦ Antibody Immunofluorescence (IF) protocol
- ♦ Antibody Immunoprecipitation (IP) protocol
- ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol
Protein&peptide usage suggestions
Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.
- ♦ Handling and storage suggestion for peptides and protein
- ♦ Cytokines and growth factors for cell culture application
Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
We hope this will be helpful for your research work. Please let us know through firstname.lastname@example.org if you need more information or support.
Inorganic Self-Assembled Bioactive Artificial Proto-Osteocells Inducing Bone Regeneration
Shi M, Yang R, Li Q, et al. ACS Appl Mater Interfaces. 2018, 10(13): 10718-10728.
A "Driver Switchover" Mechanism of Influenza Virus Transport from Microfilaments to Microtubules
Zhang LJ, Xia L, Liu SL, et al. ACS Nano. 2018, 12(1): 474-484.
Quantitative Proteomics Reveals the Regulatory Networks of Circular RNA CDR1as in Hepatocellular Carcinoma Cells
Yang X, Xiong Q, Wu Y, et al. J Proteome Res. 2017 6;16(10): 3891-3902.
Neuroglobin boosts axon regeneration during ischemic reperfusion via p38 binding and activation depending on oxygen signal
Xiong XX, Pan F, Chen RQ, et al. Cell Death Dis, 2018, 9(2): 163.
Silencing PPA2 inhibits human epithelial ovarian cancer metastasis by suppressing the Wnt/β-catenin signaling pathway
Niu H, Zhou W, Xu Y, et al. Oncotarget, 2017, 8(44): 76266-76279.
PD-L1 confers glioblastoma multiforme malignancy via Ras binding and Ras/Erk/EMT activation
Qiu XY, Hu DX, Chen WQ, et al. Biochim Biophys Acta, 2018, 1864(5 Pt A): 1754-1769.
Quantitative Proteomics Identified TTC4 as a TBK1 Interactor and a Positive Regulator of SeV-Induced Innate Immunity
Shang J, Xia T, Han QQ, et al. Proteomics, 2018, 18(2).
Probiotic Mixture Golden Bifido Prevents Neonatal Escherichia coli K1 Translocation via Enhancing Intestinal Defense
Zeng Q, He X, Puthiyakunnon S, et al. Front Microbiol, 2017, 8: 1798.
Perfluorohexadecanoic acid increases paracellular permeability in endothelial cells through the activation of plasma kallikrein-kinin system
Liu QS, Hao F, Sun Z, et al. Chemosphere, 2018, 190: 191-200.
Peli1 Contributions in Microglial Activation, Neuroinflammatory Responses and Neurological Deficits Following Experimental Subarachnoid Hemorrhage
Huang XP, Peng JH, Pang JW, et al. Front Mol Neurosci, 2017, 10: 398.
Biphasic activation of nuclear factor-κB and expression of p65 and c-Rel following traumatic neuronal injury
Zhang H, Zhang D, Li H, et al. Int J Mol Med, 2018, 41(6): 3203-3210.
IL‐6 increases podocyte motility via MLC‐mediated focal adhesion impairment and cytoskeleton disassembly
He F F, Bao D, Su H, et al. J Cell Physiol, 2018.
Curc-mPEG454, a PEGylated Curcumin Derivative, Improves Anti-inflammatory and Antioxidant Activities: a Comparative Study
Cheng F, Chen Y, Zhan Z, et al. Inflammation, 2018, 41(2): 579-594.
Altered expression of mTOR and autophagy in term normal human placentas
Zhang Q X, Na Q and Song W Rom J Morphol Embryol, 2017; 58(2): 517-526.
Neuroprotective Effect of β-Caryophyllene on Cerebral Ischemia-Reperfusion Injury via Regulation of Necroptotic Neuronal Death and Inflammation: In Vivo and in Vitro
Yang M, Lv Y, Tian X, et al. Front Neurosci, 2017, 26; 11: 583.
Trps1 is associated with the multidrug resistance of lung cancer cell by regulating MGMT gene expression
Liu H, Liao Y, Tang M, et al. Cancer Med, 2018.
Endothelial cell SHP-2 negatively regulates neutrophil adhesion and promotes transmigration by enhancing ICAM-1-VE-cadherin interaction
Yan M, Zhang X, Chen A, et al. FASEB J, 31(11): 4759-4769.
Digital Single Virus Electrochemical Enzyme-Linked Immunoassay for Ultrasensitive H7N9 Avian Influenza Virus Counting
Wu Z, Guo WJ, Bai YY, et al. Anal Chem, 2018, 90(3): 1683-1690.
SGK1 inhibition induces autophagy-dependent apoptosis via the mTOR-Foxo3a pathway
Liu W, Wang X, Liu Z, et al. Br J Cancer, 10; 117(8): 1139-1153.
Integrated Proteomic and Transcriptomic Analysis Reveals Long Noncoding RNA HOX Transcript Antisense Intergenic RNA (HOTAIR) Promotes Hepatocellular Carcinoma Cell Proliferation by Regulating Opioid Growth Factor Receptor (OGFr)
Wu Y, Xiong Q, Li S T, et al. MOLECULAR & CELLULAR PROTEOMICS, 2018, 17: 146-159.
Decidual Macrophage Functional Polarization during abnormal Pregnancy due to Toxoplasma gondii: role for lilrB4
Li Z D, Zhao M D, Li T, et al. Frontiers in Immunology, 2017.
The Role of Pannexin3-Modified Human Dental Pulp-Derived Mesenchymal Stromal Cells in Repairing Rat Cranial Critical-Sized Bone Defects
Song F, Sun H, Huang L, et al. Cellular Physiology and Biochemistry, 2017, 44: 2174–2188.
Electroacupuncture at ST36 Increases Bone Marrow-Derived Interstitial Cells of Cajal via the SDF-1/CXCR4 and mSCF/Kit-ETV1 Pathways in the Stomach of Diabetic Mice
Zhao J, An J and Liu S Evidence-Based Complementary and Alternative Medicine, 2018.
Bisphenol A and estrogen induce proliferation of human thyroid tumor cells via an estrogen-receptor-dependent pathway
Zhang T H, Wei F, Zhang J, et al. Archives of Biochemistry and Biophysics, 2017, 633: 29-39.
Apolipoprotein E-Mimetic Peptide COG1410 Promotes Autophagy by Phosphorylating GSK-3 in Early Brain Injury Following Experimental Subarachnoid Hemorrhage
Lin X S, Peng J H, Pang J W, et al. Front, Neurosci, 2018, 12(127).
Coaxial 3D bioprinting of self-assembled multicellular heterogeneous tumor fibers
Dai, Xingliang, et al. Scientific Reports 7 (2017).
Dihydromyricetin modulates p62 and autophagy crosstalk with the Keap-1/Nrf2 pathway to alleviate ethanol-induced hepatic injury
Qiu, Ping, et al. Toxicology Letters 274 (2017): 31-41.
Anti-c-Met antibody bioconjugated with hollow gold nanospheres as a novel nanomaterial for targeted radiation ablation of human cervical cancer cell
Liang, Ying, et al. Oncology Letters 14.2 (2017): 2254-2260.
Klotho suppresses tumor progression via inhibiting IGF-1R signaling in T‑cell lymphoma
Zhou, Xiangxiang, et al. Oncology Reports (2017).
Ten-Eleven Translocation-2 (Tet2) Is Involved in Myogenic Differentiation of Skeletal Myoblast Cells in Vitro
Zhong, Xia, et al. Scientific Reports 7 (2017): 43539.
Evaluation of PFOS-mediated neurotoxicity in rat primary neurons and astrocytes cultured separately or in co-culture
Li, Zhenwei, et al. Toxicology in Vitro 38 (2017): 77-90.
The mechanically activated p38/MMP-2 signaling pathway promotes bone marrow mesenchymal stem cell migration in rats
Yang, Zihui, et al. Archives of Oral Biology 76 (2017): 55-60.
Role of Wnt/β-catenin signaling pathway in the repair of intestinal mucosa associated with crypt stem cell in a rat model of abdominal compartment syndrome
Lu T, Luo H, Liu J, et al. Int J Clin Exp Pathol, 2017, 10(2): 2351-2362
Application of a microfluidic-based perivascular tumor model for testing drug sensitivity in head and neck cancers and toxicity in endothelium
Jin, Dong, et al. Rsc Advances 6.35(2016):29598-29607.
Olfactory Ensheathing Cell-Conditioned Medium Reverts Aβ 25–35 -Induced Oxidative Damage in SH-SY5Y Cells by Modulating the Mitochondria-Mediated Apoptotic Pathway
Fu, Qing-Qing, et al. Cellular and Molecular Neurobiology (2016): 1-12.
Oocyte Vitellogenesis Triggers the Entry of Yeast-Like Symbionts Into the Oocyte of Brown Planthopper (Hemiptera: Delphacidae)
Nan, Guo-Hui, et al. Annals of the Entomological Society of America 109.5 (2016): 753-758.
Inhibition of Blood-Brain Barrier Disruption by an Apolipoprotein E-Mimetic Peptide Ameliorates Early Brain Injury in Experimental Subarachnoid Hemorrhage
Pang J, Chen Y, Kuai L, et al. Translational Stroke Research, 2016: 1-16
Quantitative Proteomics Analysis Reveals Novel Insights into Mechanisms of Action of Long Noncoding RNA Hox Transcript Antisense Intergenic RNA (HOTAIR) in HeLa Cells
Zheng, Peng, et al. Molecular & Cellular Proteomics 14.6 (2015): 1447-1463.
Ricin-B-lectin enhances microsporidia Nosema bombycis infection in BmN cells from silkworm Bombyx mori.
Liu H, Li M, Cai S, et al. Acta Biochimica et Biophysica Sinica, 2016.
β‐Caryophyllene protects in vitro neurovascular unit against oxygen‐glucose deprivation and re‐oxygenation‐induced injury.
Tian X, Peng J, Zhong J, et al. Journal of Neurochemistry, 2016.
Ischemic preconditioning inhibits over-expression of arginyl-tRNA synthetase gene Rars in ischemia-injured neurons.
Shen Y, Zhao H, Wang H, et al. Journal of Huazhong University of Science and Technology [Medical Sciences], 2016, 36(4): 554-557.
Wnt5a mediated canonical Wnt signaling pathway activation in orthodontic tooth movement: possible role in the tension force-induced bone formation.
Fu H D, Wang B K, Wan Z Q, et al. Journal of Molecular Histology, 2016, 47(5): 455-466.
An apoE-derived mimic peptide, COG1410, alleviates early brain injury via reducing apoptosis and neuroinflammation in a mouse model of subarachnoid hemorrhage.
Wu Y, Pang J, Peng J, et al. Neuroscience letters, 2016.
Isolation and characterization of a fatty acid-and retinoid-binding protein from the cereal cyst nematode Heterodera avenae.
Le X, Wang X, Guan T, et al. Experimental parasitology, 2016, 167: 94-102.
Blocking mPTP on Neural Stem Cells and Activating the Nicotinic Acetylcholine Receptor α7 Subunit on Microglia Attenuate Aβ-Induced Neurotoxicity on Neural Stem Cells.
Chen Q, Wang K, Jiang D, et al. Neurochemical research, 2016, 41(6): 1483-1495.
Autophagy is involved in TGF-β1-induced protective mechanisms and formation of cancer-associated fibroblasts phenotype in tumor microenvironment.
Liu F L, Mo E P, Yang L, et al. Oncotarget, 2016, 7(4): 4122.
Diosgenin and 5-Methoxypsoralen Ameliorate Insulin Resistance through ER-α/PI3K/Akt-Signaling Pathways in HepG2 Cells.
Fang K, Dong H, Jiang S, et al. Evidence-Based Complementary and Alternative Medicine, 2016, 2016.
Lipid reduces GLP-1 production via inhibiting NF-kappa B p65 and IL-6 in intestinal L cells and pancreatic alpha cells.
Wang X, Liu J, Jin T, et al. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY, 2016, 9(6): 6224-6230.
sodium butyrate down-regulates tristetraprolin-mediated cyclin B1 expression independent of the formation of processing bodies.
Zheng X T, Xiao X Q. The international journal of biochemistry & cell biology, 2015, 69: 241-248.
Expression of Pannexin3 in human odontoblast-like cells and its hemichannel function in mediating ATP release.
Fu D, Song F, Sun H, et al. Archives of oral biology, 2015, 60(10): 1510-1516.
LGR5 Promotes Breast Cancer Progression and Maintains Stem-Like Cells Through Activation of Wnt/β-Catenin Signaling.
Yang L, Tang H, Kong Y, et al. Stem Cells, 2015, 33(10): 2913-2924.
The developmental neurotoxicity of polybrominated diphenyl ethers: Effect of DE‐71 on dopamine in zebrafish larvae.
Wang X, Yang L, Wu Y, et al. Environmental Toxicology and Chemistry, 2015, 34(5): 1119-1126.