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Human Mucin-19 (MUC19) ELISA Kit

Human Mucin-19 (MUC19) ELISA Kit

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Specification

Product name Human Mucin-19 (MUC19) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Mucin-19 (MUC19) ELISA Kit employs a two-site sandwich ELISA to quantitate MUC19 in samples. An antibody specific for MUC19 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMUC19 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC19 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC19 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MUC19; FLJ35746; mucin 19

Product Properties

Kit components • Human Mucin-19 microplate
• Human Mucin-19 standard
• Human Mucin-19 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Mucin-19 (MUC19) ELISA Kit has high sensitivity and excellent specificity for detection of Human MUC19. No significant cross-reactivity or interference between Human MUC19 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MUC19 encodes a member of the gel-forming mucin protein family. Mucin family members are glycoproteins that have tandem repeats which are extensively O-glycosylated. The structural features of mucin proteins are responsible for the gel-like properties of mucus. Mucin-19 may be involved in disruption of the ocular surface in Sjogren syndrome.
Gene ID 283463
Alternative MUC19; FLJ35746; mucin 19
Accession Q7Z5P9

Image & description

Fig.1. Human Mucin-19 (MUC19) Standard Curve.

Fig.1. Human Mucin-19 (MUC19) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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