CheKine™ Micro Uric Acid Assay Kit: Confronting the Microsample Uric Acid Detection Crisis
Uric Acid: The Double-Edged Sword of Purine Metabolism
Uric acid (UA) is a critical biomarker for gout, kidney stones, and metabolic syndrome[reference:69]. Yet measuring it in microsamples—10 µL capillary blood from a toddler with suspected Lesch-Nyhan syndrome, or a 5 mg kidney biopsy core from a diabetic patient—has been a persistent challenge[reference:70]. Traditional kits either guzzle sample volume or drown in noise[reference:71].
Abbkine's CheKine™ Micro Uric Acid (UA) Assay Kit (KTB1510) turns microsample uric acid quantification into a reliable, low-stress process[reference:72].
Why Abbkine KTB1510 Leads the Field
| Feature | Abbkine KTB1510 | Traditional UA Assays |
|---|---|---|
| Detection Principle | Uricase-catalyzed colorimetric (505 nm)[reference:73] | Variable; often less specific |
| Sample Types | Animal tissues, serum, plasma, urine[reference:74] | Limited compatibility |
| Specificity | High; uricase specifically catalyzes UA to allantoin[reference:75] | Often cross-reacts with other purines |
| Sample Volume | Microscale—minimal required | Large volumes (50+ µL) |
| Anti-Interference | Robust; designed for complex biological matrices[reference:76] | Often compromised |
| Storage | 6 months at 4°C, protected from light[reference:77] | Shorter shelf life |
The Enzymatic Specificity Advantage
The assay principle is elegant and specific: Uricase catalyzes UA to produce allantoin, CO₂, and H₂O₂; H₂O₂ oxidizes Fe²⁺ in potassium ferrocyanide to produce Fe³⁺; Fe³⁺ further condenses with phenol and 4-aminoantipyrine to form red quinone compounds with a characteristic absorption peak at 505 nm[reference:78]. The UA content is calculated by measuring the light absorption at this wavelength[reference:79].
Applications Across Research Fields
- Clinical & Translational Research — gout, kidney stones, metabolic syndrome[reference:80]
- Nephrology — monitoring uric acid in kidney disease
- Cardiovascular Research — UA as a risk factor for hypertension and heart disease
- Oxidative Stress Research — UA as both antioxidant and pro-oxidant
- Insect Physiology — uric acid biosynthesis and oxidative stress homeostasis[reference:81]
Validated in Publications
The kit has been cited in a study in Acta Biomaterialia (IF: 3) on purine nucleoside phosphorylase mediating midgut physiological functions in Aedes aegypti by orchestrating uric acid biosynthesis and oxidative stress homeostasis[reference:82].
Critical Sample Handling Tips
- Fresh samples are recommended; if not assayed immediately, store at -80°C for one month[reference:83]
- Serum, plasma, or urine samples: Test directly[reference:84]
- Tissue samples: Homogenize according to the ratio of tissue weight (g) to Extraction Buffer volume (mL)[reference:85]
- Reagent I A, Reagent I B, and Standard are toxic—experiment in a fume hood[reference:86]
- Perform a pre-experiment with 2-3 samples with large expected differences before formal testing[reference:87]
The Bottom Line
For researchers who need reliable uric acid quantification from precious, limited samples—whether rare patient sera, tiny biopsies, or insect hemolymph—KTB1510 delivers the specificity, sensitivity, and microscale efficiency that modern research demands[reference:88].
👉 Quantify uric acid with confidence. View Product
Prev：This is the latest article
