CheKine™ Micro Glucose-6-Phosphate (6PG) Detection Kit: Your Top Questions Answered

What is glucose-6-phosphate (6PG) and why measure it?

Glucose-6-phosphate (6PG) is a central metabolic intermediate found widely in animals, plants, and microorganisms[reference:17]. It sits at the crossroads of two major pathways:

• Glycolysis — the first step of glucose metabolism[reference:18]
• Pentose phosphate pathway (PPP) — the primary route for generating NADPH[reference:19]

6PG can also be converted to glycogen or starch for storage[reference:20]. Measuring 6PG content provides insights into glycolytic flux, PPP activity, and cellular energy status.

How does the detection work?

The kit employs a simple enzymatic detection system: Glucose-6-phosphate dehydrogenase catalyzes the conversion of 6PG and NADP⁺ to 6-phosphogluconate and NADPH[reference:21]. NADPH then reduces WST-8 to an orange-yellow formazan product under the action of 1-mPMS[reference:22]. The absorbance at 450 nm is directly proportional to 6PG content.

What sample types are compatible?

The kit is compatible with a wide range of samples:

• Animal and plant tissues
• Bacteria and cells
• Serum, plasma, and other fluids[reference:23]

Do I need to prepare samples fresh?

Yes. Fresh samples are necessary for good results[reference:24]. If you cannot perform the assay immediately, complete the sample preparation step before storing[reference:25]. Samples can be stored at -80°C for up to one month if not assayed immediately[reference:26].

How should I handle the standards?

It is recommended to perform several dilutions of your sample to ensure readings fall within the standard value range[reference:27]. The kit includes a Standard for accurate quantification[reference:28].

What is the storage condition?

Store the kit at -20°C for 6 months, protected from light[reference:29].

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