| Product name | CheKine™ Micro Glucose-6-Phosphate (6PG) Content Detection Kit |
| SampleType | Animal and plant tissues, bacteria and cells, serum (plasma), or other fluids. |
| Alternative | Glucose-6-Phosphate (6PG) |
| Kit components | •Extraction Buffer •Reagent I •Reagent Ⅱ •Reagent Ⅲ •Standard |
| Features & Benefits | The verified samples are complete in type and easy to operate． |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples. |
| Storage instructions | Storage at 4°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Glucose-6-phosphate (6PG), also known as Glucose 6-phosphate, is an intermediate product of glycolysis and pentose phosphate pathway, which is widely found in animals, plants and microorganisms. In the first step of glycolysis, glucose is catalyzed by hexokinase to form glucose-6-phosphate, which is then catalyzed by phosphate glucose isomerase to form fructose-6-phosphate to continue the other steps of glycolysis. However, in the pentose phosphate pathway, 6PG is its first substrate, and this process is also the main way to generate NADPH. In addition, 6PG can also be converted to glycogen or starch and stored. Glucose-6-phosphate dehydrogenase catalyzes the formation of glucose-6-phosphate and NADPH from 6PG and NADP+. NADPH can make WST-8 orange-yellow under the action of 1-mPMS, and the content of 6PG can be calculated by measuring the absorbance value at 450 nm. |
| Alternative | Glucose-6-Phosphate (6PG) |
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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