Rat Glutathione Peroxidase (GSH-PX) ELISA Kit — FAQ for Oxidative Stress Researchers

What makes Abbkine's Rat GSH-PX ELISA Kit stand out for oxidative stress research?

Glutathione peroxidase (GSH-PX) is a critical antioxidant enzyme that protects cells from oxidative damage by reducing lipid hydroperoxides and hydrogen peroxide. For researchers studying oxidative stress, aging, neurodegeneration, or cardiovascular disease, accurate GSH-PX quantification is non-negotiable. Abbkine's Rat Glutathione Peroxidase (GSH-PX) ELISA Kit (KTE101141) delivers exactly that — with a detection limit of just 0.1 pmol/mL and a calibration range spanning 3–48 pmol/mL. Whether you're working with serum, plasma, or cell culture supernatants, this kit is built for precision.

How does the sandwich ELISA format ensure accurate GSH-PX detection?

This kit employs a two-site sandwich ELISA format — the gold standard for quantitative protein detection. A GSH-PX-specific antibody is pre-coated onto the microplate. When you add standards and samples, any GSH-PX present binds to the immobilized antibody. After washing away unbound substances, an HRP-Conjugated Rat GSH-PX detection antibody is added. Following another wash, a chromogen solution is introduced — color develops in proportion to the amount of GSH-PX captured. The result? A colorimetric readout that's both sensitive and specific. No significant cross-reactivity means you can trust your data.

What sample types can I use with this kit?

Flexibility matters in the lab. This kit is validated for:

• Serum
• Plasma
• Cell culture supernatants
• Other biological fluids

Whether you're analyzing tissue homogenates, conditioned media, or systemic circulation samples, this kit adapts to your experimental design.

What's the detection range and sensitivity?

That's sub-picomolar sensitivity — essential for detecting GSH-PX in samples where expression may be low, such as in certain cell lines or diluted biological fluids.

How should I store and handle the kit?

Proper storage ensures reproducibility:

• Unopened kit: Store at 2–8°C
• Opened kit: Follow protocol-specific storage instructions
• Shipping: Gel pack with blue ice maintains temperature integrity during transit

Critical handling tips from the manufacturer:

• Allow all reagents to warm to room temperature for at least 30 minutes before opening
• Pre-rinse pipette tips with reagent and use fresh tips for each sample, standard, and reagent
• Mix thoroughly — use a low-frequency oscillator or gentle hand shaking every 10 minutes
• Run samples and standards in duplicate or triplicate
• Keep unused wells desiccated at 4°C in the provided sealed bag

Is cross-reactivity a concern?

Not with this kit. Abbkine has validated that no significant cross-reactivity or interference occurs between rat GSH-PX and analogues. You get clean data without spurious signals from related proteins.

What applications is this kit suitable for?

This kit is designed for research use only — not for human or clinical diagnosis. Applications include:

• Oxidative stress research
• Aging and longevity studies
• Neurodegenerative disease models (Alzheimer's, Parkinson's)
• Cardiovascular research
• Toxicology and drug safety assessment
• Antioxidant compound screening

Why choose Abbkine's GSH-PX ELISA Kit over competitors?

• Proven sandwich ELISA format for quantitative accuracy
• 0.1 pmol/mL LOD — among the most sensitive on the market
• Broad sample compatibility (serum, plasma, cell culture supernatants)
• No significant cross-reactivity
• Convenient storage (2–8°C) and blue ice shipping
• Complete kit components — no hidden reagents to source separately

📌 Reference Link: Rat Glutathione Peroxidase (GSH-PX) ELISA Kit — KTE101141