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There are two bottles of lysis buffer in this kit, respectively denatured protein lysates and non-denatured protein lysates. How to apply them reasonably in IP experiment?

Date:2022-03-10 Views:55

In an IP validation experiment, the Input samples were directly used for SDS-PAGE and WB experiments and could be treated with denatured lysis buffer. The samples used in the IP/ CO-IP experimental operation be treated with non-denatured lysis buffer.

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