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Promotion of the cell status detection kits

Date:2021-07-15 Views:276

Do you have any trouble in the process of exploring the cell's proliferation, aging, and apoptosis? Perhaps there are some concerns about that weak fluorescence will interfere with your judgment?  Or did the cumbersome steps block your thoughts?

  • Annexin V of AbFluor™ - Increases Fluorescence Intenacity by 30% or More!  
  • It saves half of the operation time with the EDU Kit!

During this summer, the Abbkine's cell state assay kits will "witness" the vitality, apoptosis and aging in the world of cells with you!

 

Promotion participants: Authorized Distributors Overseas.

Promotion time: From now to 30th September, 2021.

Promotion products: Cell status detection in the attached list (Table 1).

Promotion Discount: 55% off.

 

Table 1:

Product Name Cat. No Size

List price ($)

Discounted price ($)

Cell Counting Kit-8 (CCK-8) KTA1020 200T/1000T/10000T 19/69/529 9/31/238
Annexin V-AbFluor™ 405 Apoptosis Detection Kit KTA0001 20T/50T/100T 79/159/269 36/71/121
Annexin V-AbFluor™ 488 Apoptosis Detection Kit KTA0002 20T/50T/100T 79/159/269 36/71/121
Annexin V-AbFluor™ 555 Apoptosis Detection Kit KTA0003 20T/50T/100T 79/159/269 36/71/121
Annexin V-AbFluor™ 647 Apoptosis Detection Kit KTA0004 20T/50T/100T 79/159/269 36/71/121
Live and Dead Cell Double Staining Kit KTA1001 100T/500T/2000T 79/239/589 36/108/265
Mitochondrial Membrane Potential Assay Kit (JC-1) KTA4001 20T/100T/500T 49/149/449 22/67/202
LDH Cytotoxicity Assay Kit KTA1030 96T/480T 89/199 40/90
TUNEL Apoptosis Detection Kit (Green Fluorescence) KTA2010 20T/50T/100T 189/319/499 85/144/225
TUNEL Apoptosis Detection Kit (Orange Fluorescence) KTA2011 20T/50T/100T 189/319/499 85/144/225
Cell Cycle Staining Kit KTA2020 50T/100T/500T 69/109/319 31/49/144
Cell Proliferation EdU Image Kit (Green Fluorescence) KTA2030 50T/100T/500T 69/119/189 31/54/85
Cell Proliferation EdU Image Kit (Orange Fluorescence) KTA2031 50T/100T/500T 69/119/189 31/54/85
Caspase-1 Assay Kit (Colorimetric) KTA3020 20T/50T/100T 89/159/289 40/71/130
Caspase-2 Assay Kit (Colorimetric) KTA3021 20T/50T/100T 89/159/289 40/71/130
Caspase-3 Assay Kit (Colorimetric) KTA3022 20T/50T/100T 89/159/289 40/71/130
Caspase-4 Assay Kit (Colorimetric) KTA3023 20T/50T/100T 89/159/289 40/71/130
Caspase-6 Assay Kit (Colorimetric) KTA3024 20T/50T/100T 89/159/289 40/71/130
Caspase-8 Assay Kit (Colorimetric) KTA3025 20T/50T/100T 89/159/289 40/71/130
Caspase-9 Assay Kit (Colorimetric) KTA3026 20T/50T/100T 89/159/289 40/71/130
Senescence β-Galactosidase Staining Kit KTA3030 100T 89 40

 

Experimental results:

Annexin V-Abfluor ™ 488 Apoptosis Detection Kit

Annexin V-AbFluor™ 488(1)

Fig 1. Hela cells were induced with camptothecin for 24 hours and stained with Annexin V- AbFluor™ 488 Apoptosis Detection Kit. The cells were in late-stage apoptotic/necrotic with both Annexin V- AbFluor™ 488 and PI staining (green membrane with red fragmented nucleus).

Annexin V-AbFluor™ 488(2)

Fig 2. Hela cells were induced with camptothecin of 4 different concentration(0, 1:8000, 1:4000, 1:2000, 1:1000) for 24 hours and stained with Annexin V- AbFluor™ 488 Apoptosis Detection Kit. The combination of AbFluor™ 488 and propidium iodide allows for the distinction between early apoptotic cells (Annexin V- AbFluor™ 488 positive), late apoptotic and/or necrotic cells (Annexin V- AbFluor™ 488 and propidium iodide positive), and viable cells (unstained).

 

TUNEL Apoptosis Detection Kit(Orange Fluorescence)

TUNEL Apoptosis Detection Kit (Orange Fluorescence)

Fig 3. Fluorescence images using Abbkine TUNEL Apoptosis Detection Kit (Orange Fluorescence) in HeLa cells with the treatment of 10 U/mL Dnase I for 10min show the TUNEL (red) and DAPI (blue) staining. The final image (C) is created by overlaying the image (A) onto the original image (B) to provide a visual assessment of counted TUNEL and DAPI+ cells.

 

TUNEL Apoptosis Detection Kit (Green Fluorescence)

TUNEL Apoptosis Detection Kit (Green Fluorescence)

Fig 4. Fluorescence images using Abbkine TUNEL Apoptosis Detection Kit (Green Fluorescence) in HeLa cells with the treatment of 10 U/mL Dnase I for 10min show the TUNEL (Green) and DAPI (blue) staining. The final image (C) is created by overlaying the image (A) onto the original image (B) to provide a visual assessment of counted TUNEL and DAPI+ cells.

 

References:

  1. Ocular surface repair using decellularized porcine conjunctiva. Acta Biomater. 2020 Jan 1;101:344-356. (IF= 7.242)
  2. Polyphyllin VI Induces Caspase-1-Mediated Pyroptosis via the Induction of ROS/NF-κB/NLRP3/GSDMD Signal Axis in Non-Small Cell Lung Cancer. Cancers (Basel). 2020 Jan 13;12(1):193. (IF=6.126)
  3. P2X7 Receptor Induces Pyroptotic Inflammation and Cartilage Degradation in Osteoarthritis via NF-κB/NLRP3 Crosstalk. Oxid Med Cell Longev. 2021 Jan 16;2021:8868361. (IF=076)
  4. Dihydromyricetin alleviates doxorubicin-induced cardiotoxicity by inhibiting NLRP3 inflammasome through activation of SIRT1. Biochem Pharmacol. 2020 May;175:113888. (IF=5.009)
  5. Integrin β3 promotes cardiomyocyte proliferation and attenuates hypoxia-induced apoptosis via regulating the PTEN/Akt/mTOR and ERK1/2 pathways. Int J Biol Sci. 2020 Jan 14;16(4):644-654. (IF=4.858)
  6. Silencing hsa_circRNA_0008035 exerted repressive function on osteosarcoma cell growth and migration by upregulating microRNA-375. Cell Cycle. 2020 Sep;19(17):2139-2147. (IF=4.565)
  7. Circular RNA SMARCA5 functions as an anti-tumor candidate in colon cancer by sponging microRNA-552. Cell Cycle. 2021 Apr;20(7):689-701. (IF= 4.565)
  8. Engeletin suppresses lung cancer progression by inducing apoptotic cell death through modulating the XIAP signaling pathway: A molecular mechanism involving ER stress. Biomed Pharmacother. 2020 Aug;128:110221. (IF= 4.545)
  9. The main anthocyanin monomer of Lycium ruthenicum Murray induces apoptosis through the ROS/PTEN/PI3K/Akt/caspase 3 signaling pathway in prostate cancer DU-145 cells. Food Funct. 2021 Feb 21;12(4):1818-1828. (IF= 171)
  10. Comparison of IVF and IVM outcomes in the same patient treated with a modified IVM protocol along with an oocytes-maturing system containing melatonin: A pilot study. Life Sci. 2021 Jan 1;264:118706. (IF=3.647)

Note: The final interpretation right of this event belongs to Abbkine

 

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