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Human Metallothionein-2 (MT-2) ELISA Kit

Human Metallothionein-2 (MT-2) ELISA Kit

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Specification

Product name Human Metallothionein-2 (MT-2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Metallothionein-2 (MT-2) ELISA Kit employs a two-site sandwich ELISA to quantitate MT2A in samples. An antibody specific for MT2A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMT2A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MT2A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MT2A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MT2A; MT2;

Product Properties

Kit components • Human Metallothionein-2 microplate
• Human Metallothionein-2 standard
• Human Metallothionein-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Metallothionein-2 (MT-2) ELISA Kit has high sensitivity and excellent specificity for detection of Human MT2A. No significant cross-reactivity or interference between Human MT2A and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MT2A (Metallothionein 2A) is a Protein Coding gene, and is affiliated with the lncRNA class. Diseases associated with MT2A include Inverted Papilloma and Scrapie. Among its related pathways are Interferon gamma signaling and Metabolism. GO annotations related to this gene include drug binding. An important paralog of this gene is MT1G. Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Gene ID 4502
Alternative MT2A; MT2;
Accession P02795

Image & description

Fig.1. Human Metallothionein-2 (MT-2) Standard Curve.

Fig.1. Human Metallothionein-2 (MT-2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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