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CheKine™ Micro Acetyl Coenzyme A (Acetyl-CoA) Assay Kit

CheKine™ Micro Acetyl Coenzyme A (Acetyl-CoA) Assay Kit

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Specification

Product name CheKine™ Micro Acetyl Coenzyme A (Acetyl-CoA) Assay Kit
Applications notes CheKine™ Micro Acetyl Coenzyme A (Acetyl-CoA) Assay Kit provides a simple, sensitive, rapid colorimetric Acetyl-CoA detection method, which is compatible to various biological samples, especially animals or plant tissues or cell cultures (adherent or suspension). The detection principle is that malate dehydrogenase can catalyze malic acid and NAD to produce oxaloacetic acid and NADH. citrate synthase can catalyze Acetyl-CoA and oxaloacetic acid to produce citric acid and CoA. Based on the coupling reaction of malate dehydrogenase and citrate synthase, Acetyl-CoA content is is proportional to the generating rate of NADH . NADH has a special absorption peak at 340 nm, and the calculation of the absorbance value of 340 nm can be obtained the content of Acetyl-CoA.

Product Properties

Kit components • Extraction Buffer
• ReagentⅠ
• Reagent Ⅱ
• Reagent Ⅲ
• Reagent Ⅳ
• Standard
Features & Benefits • simple, sensitive, rapid colorimetric Acetyl-CoA detection method.
• compatible to various biological samples, especially animal or plant tissues or cell cultures (adherent or suspension).
• Provides detailed sample preparation and results calculation methods.
Usage notes • Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal.
• Avoid bubbles while mixing or redissolving components.
• Change pipette tips frequently to avoid cross contamination between components.
• Ensure that all components and equipment are at the proper temperature before starting the experiment.
• All samples and reagents should be on ice to avoid denaturation and deactivation .
• Please to extract Acetyl-CoA from fresh samples in order to ensure the enzyme activity .
• Please reduce the tissues weight if the OD values is too high,or reduce the volume of Extraction Buffer if the OD values is too low .
Storage instructions Storage at -20°C and protected from light upon receipt. Kit has a storage time of 6 months form receipt.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Acetyl-CoA is widely present in animals, plants, microorganisms and cultured cells. It is an important intermediate metabolite produced during the metabolism of biometrics. In vivo energy substance metabolism is a hinging material. The three major nutrients --glucose, lipid, protein which could be via acetyl coenzyme A to form a common metabolic pathway - tricarboxylic acid circulation and phosphorylation. Through this path, Acetyl-CoA thoroughly could be oxidize carbon dioxide and water, and release energy for ATP synthesis. Further, Acetyl-CoA a is a precursor material while synthetic fatty acid, ketone body , and Cholesterol and its derivatives .

Image & description

Fig. Standard Curve of acetyl coenzyme A assay. The x-axis is OD340 and the y-axis is acetyl coenzyme A concentration (nmol/mL).

Fig. Standard Curve of acetyl coenzyme A assay. The x-axis is OD340 and the y-axis is acetyl coenzyme A concentration (nmol/mL).

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