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Vinculin Polyclonal Antibody

Vinculin Polyclonal Antibody

Views(442) Publications(0) Catalog no(ABP52701)
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Specification

Product name Vinculin Polyclonal Antibody
Immunogen Synthesized peptide derived from human Vinculin around the non-phosphorylation site of Y821
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative VCL; Vinculin; Metavinculin

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
Gene ID 7414
Alternative VCL; Vinculin; Metavinculin
Others Vinculin Polyclonal Antibody detects endogenous levels of Vinculin protein.
Accession P18206
Observed Band(KD) 123

Image & description

Fig. Western Blot analysis of various cells using Vinculin Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig. Western Blot analysis of various cells using Vinculin Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

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