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DDX3 Polyclonal Antibody

DDX3 Polyclonal Antibody

Views(61) Publications(0) Catalog no(ABP53986)
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Specification

Product name DDX3 Polyclonal Antibody
Immunogen Synthesized peptide derived from human DDX3 around the non-phosphorylation site of Y69
Host Rabbit
Reactivity Human, Mouse
Applications ELISA, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative DDX3X; DBX; DDX3; ATP-dependent RNA helicase DDX3X; DEAD box protein 3; X-chromosomal; DEAD box, X isoform; Helicase-like protein 2; HLP2; DDX3Y; DBY; ATP-dependent RNA helicase DDX3Y; DEAD box protein 3, Y-chromosomal

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background DEAD-box helicase 3, X-linked encoded by DDX3X is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants.
Gene ID 1654/8653
Alternative DDX3X; DBX; DDX3; ATP-dependent RNA helicase DDX3X; DEAD box protein 3; X-chromosomal; DEAD box, X isoform; Helicase-like protein 2; HLP2; DDX3Y; DBY; ATP-dependent RNA helicase DDX3Y; DEAD box protein 3, Y-chromosomal
Others DDX3 Polyclonal Antibody detects endogenous levels of DDX3 protein.
Accession O00571/O15523
Observed Band(KD) 73

Image & description

Fig.1. Western Blot analysis of Jurkat cells using DDX3 Polyclonal Antibody diluted at 1:2000.

Fig.1. Western Blot analysis of Jurkat cells using DDX3 Polyclonal Antibody diluted at 1:2000.

Fig.2. Western Blot analysis of L929 MCF7 HELA cells using Antibody diluted at 1:2000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Western Blot analysis of L929 MCF7 HELA cells using Antibody diluted at 1:2000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

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