| Product name | CheKine™ Aspartate Aminotransferase (AST/GOT) Activity Assay Kit |
| SampleType | Animal and Plant Tissues, Cells or Bacteria, Plasma, Serum or other Liquid samples |
| Alternative | Malic Acid |
| Kit components | •Reagent Ⅰ •Reagent Ⅱ •Reagent Ⅲ |
| Features & Benefits | The verified samples are complete in type and easy to operate. |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. |
| Storage instructions | Storage at 4°C upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Aspartate aminotransferase (AST, formerly known as glutamic-oxaloacetic transaminase, GOT, EC 2.6.1.1) is widely distributed in animal and plant tissues, predominantly in the liver, myocardium, skeletal muscle and kidney. Upon injury and rupture of tissue cells, large quantities of the enzyme are released into body fluids. Therefore, AST activity serves as an important biochemical indicator for evaluating the degree of tissue-cell damage. CheKine™Aspartate Aminotransferase (AST/GOT) Activity Assay Kit (IFCC Rate Method) can be used to detect biological samples such as animal and plant tissues, cells or bacteria, plasma, serum or other liquid samples. AST in the sample catalyzes the transamination reaction between L-aspartate and α-ketoglutarate to produce oxaloacetate and L-glutamate. Malate dehydrogenase (MDH) reduces the generated oxaloacetate to L-malate, accompanied by the consumption of NADH and formation of NAD+. NADH exhibits strong characteristic ultraviolet absorption at 340 nm, whereas NAD+ shows negligible absorption at this wavelength. AST activity can be calculated by measuring the decreasing rate of absorbance at 340 nm. |
| Alternative | Malic Acid |
Fig.CheKine™ Aspartate Aminotransferase (AST/GOT) Activity Assay Kit
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