| Product name | Human FasL Protein, His-SUMO tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Human FasL Protein (Gln130-Leu281) (P48023-1) was expressed with 6×His and SUMO tag at the N-terminus. |
| Applications | His-SUMO tag |
| Applications notes | Human |
| Activity | Measure by its ability to induce apoptosis in Jurkat cells. The ED₅₀ for this effect is <1 ng/mL. The specific activity of recombinant human FasL is > 1 x 10⁶ IU/mg. |
| Protein length | The recombinant Human FasL Protein consists of 152 amino acids and predicts a molecular mass of 29.51 kDa. |
| Preparation method | E.coli |
| Purity | > 98 % as determined by SDS-PAGE |
| Molecular Formula | 29.51 kDa |
| Alternative | fas ligand, soluble Fas Ligand (sFasL), TNFSF6, CD95L, Apo I Ligand, APTL, APT1LG1, CD178 |
| Formulation | Lyophilized from sterile PBS，pH 8.0. |
| Features & Benefits | <0.1 EU per μg of the protein as determined by the LAL method. |
| Molecular weight | 29.51 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously. |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA,10% FBS or 0.1%BSA). Please prevent freeze-thaw cycles. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Fas Ligand (FasL) is a 17.34 kDa tumor necrosis factor with 152 amino acid residues. FasL is expressed from lymphoid tissue and secreted to blood. Binding to its receptor, TNFRSF6/FAS, leads to induce apoptotic signal into cells. Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development. |
| Gene ID | 356 |
| Alternative | fas ligand, soluble Fas Ligand (sFasL), TNFSF6, CD95L, Apo I Ligand, APTL, APT1LG1, CD178 |
| Accession | P48023 |
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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