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Human UbiA prenyltransferase domain-containing protein 1 (UBIAD1) ELISA Kit

Human UbiA prenyltransferase domain-containing protein 1 (UBIAD1) ELISA Kit

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Specification

Product name Human UbiA prenyltransferase domain-containing protein 1 (UBIAD1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human UbiA prenyltransferase domain-containing protein 1 (UBIAD1) ELISA Kit employs a two-site sandwich ELISA to quantitate UBIAD1 in samples. An antibody specific for UBIAD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUBIAD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBIAD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBIAD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative UBIAD1; RP4-796F18.1; SCCD; TERE1; transitional epithelia response protein

Product Properties

Kit components • Human UbiA prenyltransferase domain-containing protein 1 microplate
• Human UbiA prenyltransferase domain-containing protein 1 standard
• Human UbiA prenyltransferase domain-containing protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human UbiA prenyltransferase domain-containing protein 1 (UBIAD1) ELISA Kit has high sensitivity and excellent specificity for detection of Human UBIAD1. No significant cross-reactivity or interference between Human UBIAD1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TERE1 expression in both superficial and muscle-invasive transitional cell carcinomas of bladder (TCCs) compared with normal human urothelium. Transfection of TERE1 into 2 human TCC cell lines inhibited cellular proliferation by 80 to 90%. In 1 line, TERE1 overexpression caused a change in morphology, with an apparent increase in intercellular contacts The deduced 338-amino acid protein has a calculated molecular mass of 36.8 kD. TERE1 has 5 putative transmembrane regions, an N-glycosylation site, and several putative phosphorylation sites. Northern blot and PCR analyses showed ubiquitous expression of 1.5- and 3-kb TERE1 transcripts. Immunohistochemical analysis of human urothelium revealed nuclear and cytoplasmic expression of TERE1.
Gene ID 29914
Alternative UBIAD1; RP4-796F18.1; SCCD; TERE1; transitional epithelia response protein
Accession Q9Y5Z9

Image & description

Fig.1. Human UbiA prenyltransferase domain-containing protein 1 (UBIAD1) Standard Curve.

Fig.1. Human UbiA prenyltransferase domain-containing protein 1 (UBIAD1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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