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Human Tyrosine hydroxylase (TH) ELISA Kit

Human Tyrosine hydroxylase (TH) ELISA Kit

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Specification

Product name Human Tyrosine hydroxylase (TH) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Tyrosine hydroxylase (TH) ELISA Kit employs a two-site sandwich ELISA to quantitate TH in samples. An antibody specific for TH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TH; DYT14; DYT5b; TYH; dystonia 14; tyrosine 3-monooxygenase

Product Properties

Kit components • Human Tyrosine hydroxylase microplate
• Human Tyrosine hydroxylase standard
• Human Tyrosine hydroxylase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Tyrosine hydroxylase (TH) ELISA Kit has high sensitivity and excellent specificity for detection of Human TH. No significant cross-reactivity or interference between Human TH and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Tyrosine hydroxylase (TH) is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in TH have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for TH.
Gene ID 7054
Alternative TH; DYT14; DYT5b; TYH; dystonia 14; tyrosine 3-monooxygenase
Accession P07101

Image & description

Fig.1. Human Tyrosine hydroxylase (TH) Standard Curve.

Fig.1. Human Tyrosine hydroxylase (TH) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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