KTE0003 | EliKine™ Estradiol ELISA Kit — Ultrasensitive Estrogen Detection for Reproductive Biology, Oncology, and Beyond

Estradiol: The Most Potent Estrogen, The Most Demanding Assay

Estradiol (E2) is the most potent and prevalent estrogen in the human body—a critical regulator of female reproductive development, menstrual cyclicity, bone metabolism, and cardiovascular health. It also plays an undeniable role in hormone-dependent cancers, including breast and endometrial carcinoma. But here is the challenge: estradiol circulates at picomolar concentrations (pmol/L). Detecting these vanishingly low levels demands an assay with exceptional sensitivity and specificity. The EliKine™ Estradiol ELISA Kit (KTE0003) rises to that challenge.

Competitive ELISA: Designed for Small Molecule Precision

Like its progesterone counterpart, the Estradiol ELISA Kit employs the competitive inhibition enzyme immunoassay technique. The microtiter plate is pre-coated with an antibody specific to estradiol. Standards or samples compete with biotin-conjugated estradiol for binding to the immobilized antibody. The more estradiol in your sample, the less biotin-conjugated estradiol binds. After washing, avidin-HRP is added, substrate is introduced, and color develops inversely proportional to estradiol concentration. This competitive format is ideally suited for small-molecule hormone quantification.

Why Sensitivity Matters in Estradiol Detection

Estradiol concentrations fluctuate dramatically across the menstrual cycle, drop to near-undetectable levels in postmenopausal women, and vary widely across species and experimental models. A detection limit that cannot capture these extremes renders your data incomplete. KTE0003 is engineered to deliver reliable quantification across the full physiological range—from the lowest basal levels to peak ovulatory surges.

Broad Species Reactivity — One Kit, Limitless Applications

With reactivity validated across Bovine, Chicken, Dog, Goat, Human, Monkey, Rabbit, Rat, Sheep, and Swine, this single kit supports everything from human clinical research to large animal veterinary studies and rodent toxicology models.

Specifications at a Glance

• Assay Type: Competitive ELISA (quantitative)
• Sample Types: Serum, Plasma, Other biological fluids
• Assay Duration: 3–4 hours
• Storage: 2–8°C unopened
• Shipping: Gel pack with blue ice

No Significant Cross-Reactivity — Specificity You Can Count On

Rigorous validation confirms no significant cross-reactivity or interference between estradiol and its structural analogues. When you measure estradiol, you measure estradiol—not cross-reacting metabolites or structurally similar compounds.

Who Needs This Kit?

• Reproductive endocrinologists studying ovarian function and cycle regulation
• Oncology researchers investigating hormone-dependent tumor biology
• Toxicologists assessing environmental estrogen mimics
• Developmental biologists exploring estrogen’s role in embryogenesis
• Pharmacologists developing SERMs and aromatase inhibitors

🔗 Reference Link: https://www.abbkine.com/product/elikine-estradiol-elisa-kit-kte0003/