| Product name | EliKine™ Estradiol ELISA Kit |
| Reactivity | Donkey, Human |
| Applications | ELISA |
| Applications notes | EliKine™ Estradiol ELISA Kit employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for estradiol and Horseradish Peroxidase (HRP) conjugated estradiol. The competitive inhibition reaction is launched between with HRP labeled estradiol and unlabeled estradiol with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of estradiol in the sample. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Other biological fluids, Plasma, Serum |
| Assay type | Competitive ELISA (quantitative) |
| Assay duration | Multiple steps standard competitive ELISA assay with a working time of 3-4 hours. It depends on the experience of the operation person. |
| Alternative | E2; 17β-estradiol; Estrogen |
| Kit components | • Estradiol microplate • Estradiol standard • HRP conjugated estradiol • Estradiol detect antibody • HRP substrate A • HRP substrate B • Stop solution • Wash buffer • Plate covers. |
| Features & Benefits | EliKine™ Estradiol ELISA Kit has high sensitivity and excellent specificity for detection of estradiol. No significant cross-reactivity or interference between estradiol and analogues was observed. |
| Calibration range | 40 pg/ml-1500 pg/ml |
| Limit of detection | 40 pg/ml |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for |
| Storage instructions | The unopened kit should be stored at 2 - 8 °C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Estradiol (E2), also spelled oestradiol, is a steroid, an estrogen, and the primary female sex hormone. It is named for and is important in the regulation of the estrous and menstrual female reproductive cycles. Estradiol is essential for the development and maintenance of female reproductive tissues such as the breasts, uterus, and vagina during puberty, adulthood, and pregnancy, but it also has important effects in many other tissues, including bone, fat, skin, liver, and the brain. While estrogen levels in men are lower compared to those in women, estrogens have essential functions in men, as well. It is found in most vertebrates and crustaceans, insects, fish, and other animal species. |
| Alternative | E2; 17β-estradiol; Estrogen |
Fig.1. Estradiol Standard Curve.
Fig.2. EliKine™ Estradiol ELISA Kit is a competitive ELISA to quantitate Estradiol in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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