KTA2011 Orange Red Fluorescence TUNEL Kit – Spectrum-Isolated Apoptosis Staining for Multiplex Immunofluorescence Co-Detection

Key Drawbacks of Single Green Channel TUNEL Detection

Green fluorescent TUNEL reagents remain popular in most labs for apoptosis detection, yet researchers frequently face spectrum overlap interference when co-staining with GFP-tagged proteins or other green fluorophore biomarkers. Spectral cross-interference blurs target signals and makes it impossible to simultaneously observe apoptotic DNA damage and intracellular protein expression within one single sample slice. Scientists studying multi-marker spatial cell profiling have to prepare duplicate slides separately, consuming more limited samples and doubling daily experimental workload. Abbkine KTA2011 solves this multiplex imaging bottleneck with unique orange-red fluorescent labeling.

Orange Fluorescence Probe System Optimized for Multi-Color Co-Staining

KTA2011 adopts orange-red fluorescent dUTP probes with fixed excitation/emission wavelengths at 555 nm/565 nm, fully separated from FITC and GFP fluorescence channels without signal overlap. The one-step premixed labeling liquid combines TdT transferase and fluorescent markers, simplifying all staining procedures while retaining ultra-high labeling specificity for apoptotic DNA nicks. The fluorescent probe only binds to broken DNA 3′-OH ends, producing zero non-specific signals from intact cell chromatin or necrotic cell debris.

Complete Reagent Portfolio Compatible with Cells & Paraffin Tissue

Identical to the KTA2010 green fluorescence kit, KTA2011 is equipped with a full reagent system covering all pretreatment and staining workflows: TdT Enzyme, 5× Equilibration Buffer, Red Label Mix, 500× DAPI solution, tissue autofluorescence quencher, Proteinase K and DNase I positive control. The matched tissue pretreatment reagents eliminate paraffin slice background noise, generating clear red apoptotic signals and distinct blue nuclear DAPI staining as verified in HeLa cell validation imaging.

Diverse Application Scenarios & Reliable Long-Term Stability

This orange-red TUNEL kit is specially designed for multi-color immunofluorescence co-staining experiments, including organoid cell lineage tracing, tumor tissue multi-marker spatial analysis and neuronal cell co-localization research. It works seamlessly with fluorescence microscopes, flow cytometry and microplate readers for quantitative apoptosis analysis. All reagents remain enzymatically stable for 12 months under -20°C cold storage, shipped with blue ice gel packs to avoid activity loss during long-distance transit. Up to 24 peer-reviewed publications have validated its consistent low-background staining performance.

Critical Safety & Usage Reminders for Lab Operators

Toxic heavy metal salts exist in the reaction equilibration buffer. Any accidental skin exposure requires immediate thorough water washing; seek professional medical treatment if reagents contact eyes or are ingested. This product is only authorized for life science basic research, and strictly prohibited for clinical in vitro diagnostic use on human samples. All operation steps must follow the detailed protocol enclosed in the kit package.
Product Link: https://www.abbkine.com/product/one-step-tunel-apoptosis-assay-kit-red-fluorescence-kta2011/