Human Tubulin beta-4 chain (TUBB4) ELISA Kit — FAQ for Cytoskeleton and Neurobiology Researchers

Human TUBB4 ELISA Kit: Your Questions Answered

Tubulin beta-4 chain (TUBB4) , also known as TUBB5 or beta-5 tubulin, is a member of the tubulin family that forms the building blocks of microtubules[reference:55]. Microtubules are essential components of the cytoskeleton, playing critical roles in cell division, intracellular transport, cell motility, and neuronal development. Mutations in TUBB4 are associated with neurological disorders, including hypomyelination with atrophy of the basal ganglia and cerebellum (H-ABC) .

Abbkine's Human Tubulin beta-4 chain (TUBB4) ELISA Kit (KTE60116) provides researchers with a reliable tool for quantifying this important cytoskeletal protein in human samples. Here are answers to the most common questions about this kit.

What is the detection principle of this TUBB4 ELISA kit?

This kit employs a two-site sandwich ELISA to quantitate TUBB4 in samples[reference:56]. An antibody specific for TUBB4 has been pre-coated onto a microplate. When you add standards and samples, any TUBB4 present binds to the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TUBB4 is added[reference:57]. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added[reference:58]. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added and color develops in proportion to the amount of TUBB4 bound[reference:59]. The color development is stopped and the intensity is measured — providing a quantitative, colorimetric readout[reference:60].

What sample types can I use?

The kit is validated for serum, plasma, cell culture supernatants, and other biological fluids[reference:61]. This flexibility makes it suitable for various experimental systems, from in vitro cell culture models to clinical research samples.

What is the sensitivity and detection range?

The calibration range and limit of detection are listed as "Please inquire" on the product page[reference:62]. Abbkine's ELISA kits are engineered for high sensitivity and excellent specificity[reference:63]. For specific sensitivity information, contact Abbkine's technical support team with your expected concentration range.

Does this kit cross-react with other tubulin isoforms?

No significant cross-reactivity or interference between human TUBB4 and analogues has been observed[reference:64]. This specificity ensures you're measuring TUBB4 specifically — without interference from other tubulin beta isoforms (TUBB, TUBB2A, TUBB3, TUBB4B, etc.)[reference:65].

How should I store and handle the kit?

• Unopened kit: Store at 2–8°C[reference:66]
• Opened kit: Follow protocol-specific storage instructions[reference:67]
• Shipping: Gel pack with blue ice maintains temperature during transit[reference:68]

Critical handling tips:

• Allow all reagents to warm to room temperature for at least 30 minutes before opening[reference:69]
• Pre-rinse pipette tips with reagent; use fresh tips for each sample, standard, and reagent[reference:70]
• Mix thoroughly — use a low-frequency oscillator or gentle hand shaking every 10 minutes[reference:71]
• Run samples and standards in duplicate or triplicate[reference:72]
• Keep unused wells desiccated at 4°C in the provided sealed bag[reference:73]

What applications is this kit suitable for?

This kit is designed for research use only — not for human or clinical diagnosis[reference:74]. Applications include:

• Cytoskeleton biology research (microtubule dynamics, cell structure)
• Neuroscience (neuronal development, axonal transport)
• Neurodevelopmental disorders (H-ABC, other TUBB4-related disorders)
• Cell division and mitosis studies
• Intracellular transport research
• Cancer research (microtubule-targeting chemotherapeutics)
• Drug development (microtubule-stabilizing and -destabilizing agents)

What's included in the kit?

A complete, ready-to-use kit[reference:75].

📌 Reference Link: Human Tubulin beta-4 chain (TUBB4) ELISA Kit — KTE60116