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EliKine™ Human IL-1β ELISA Kit — Comprehensive Review & Buying Guide

Date:2026-07-21 Views:46

EliKine™ Human IL-1β ELISA Kit: A Critical Review for Inflammation Researchers

Interleukin 1β (IL-1β) is a member of the interleukin 1 cytokine family[reference:76]. Produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE), IL-1β is an important mediator of the inflammatory response and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis[reference:77]. It is a key biomarker for inflammationautoimmune diseasesinfectious diseases, and cancer.

The EliKine™ Human IL-1β ELISA Kit (KTE6013) from Abbkine provides a sensitive, specific, and complete solution for quantifying IL-1β in human samples. With 5 publications citing this kit, it's a trusted tool in the research community[reference:78]. Here's what you need to know before buying.

What's in the Box?

ComponentIncluded
Human IL-1β microplate
Human IL-1β standard
Human IL-1β detect antibody
EliKine™ Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Everything you need — no additional reagents to purchase[reference:79].

Advantages

1. Excellent Sensitivity

With a limit of detection of 4 pg/mL and a calibration range of 7.8–500 pg/mL, this kit covers both normal and elevated IL-1β levels with outstanding sensitivity[reference:80].

2. High Specificity

No significant cross-reactivity or interference between human IL-1β and analogues has been observed[reference:81]. Your IL-1β signal is clean and specific.

3. Broad Sample Compatibility

Validated for serum, plasma, cell culture supernatants, and other biological fluids[reference:82] — versatile for diverse experimental setups.

4. Published Validation

This kit has been cited in 5 publications[reference:83] — providing real-world confidence from the research community.

5. EliKine™ Streptavidin-HRP Technology

The proprietary EliKine™ Streptavidin-HRP conjugates provide enhanced signal amplification compared to traditional HRP detection systems[reference:84].

6. Complete Kit

All components included — no hidden costs or last-minute reagent sourcing[reference:85].

7. Reliable Storage and Shipping

  • Unopened kit: store at 2–8°C[reference:86]
  • Shipped with gel pack and blue ice[reference:87]

Disadvantages

1. Human-Specific

This kit is specific to human IL-1β — not validated for mouse, rat, or other species[reference:88].

2. Research Use Only

Not intended for human or clinical diagnosis[reference:89].

3. Assay Duration

Standard sandwich ELISA with a working time of 3–5 hours[reference:90].

Who Should Buy This Kit?

✅ Buy this kit if you:

  • Study inflammation, immunology, or autoimmune diseases
  • Research infectious diseases and host-pathogen interactions
  • Investigate cancer biology (tumor microenvironment, inflammation)
  • Need to quantify human IL-1β in serum, plasma, or cell culture
  • Require high sensitivity (4 pg/mL LOD)
  • Value specificity — no cross-reactivity with IL-1α or other interleukins
  • Want a complete, ready-to-use kit
  • Appreciate EliKine™ Streptavidin-HRP amplification for better signal
  • Prefer a kit with published validation (5 citations)

❌ Consider alternatives if you:

  • Need to detect mouse or rat IL-1β (check Abbkine's other offerings)
  • Are working with clinical diagnostic samples
  • Require a faster assay (<3 hours)

Quick Specs Summary

ParameterDetail
ReactivityHuman
TargetInterleukin 1β (IL-1β)
Assay typeSandwich ELISA (quantitative)
Detection methodColorimetric (EliKine™ Streptavidin-HRP)
Calibration range7.8–500 pg/mL
Limit of detection4 pg/mL
Sample typesSerum, plasma, cell culture supernatants, biological fluids
Assay duration3–5 hours
Publications5 citations
Storage2–8°C (unopened)
ShippingGel pack with blue ice

📌 Reference Link: EliKine™ Human IL-1β ELISA Kit — KTE6013