EliKine™ Human IL-1β ELISA Kit — Comprehensive Review & Buying Guide

EliKine™ Human IL-1β ELISA Kit: A Critical Review for Inflammation Researchers
Interleukin 1β (IL-1β) is a member of the interleukin 1 cytokine family[reference:76]. Produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE), IL-1β is an important mediator of the inflammatory response and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis[reference:77]. It is a key biomarker for inflammation, autoimmune diseases, infectious diseases, and cancer.
The EliKine™ Human IL-1β ELISA Kit (KTE6013) from Abbkine provides a sensitive, specific, and complete solution for quantifying IL-1β in human samples. With 5 publications citing this kit, it's a trusted tool in the research community[reference:78]. Here's what you need to know before buying.
What's in the Box?
| Component | Included |
|---|---|
| Human IL-1β microplate | ✅ |
| Human IL-1β standard | ✅ |
| Human IL-1β detect antibody | ✅ |
| EliKine™ Streptavidin-HRP | ✅ |
| Standard diluent | ✅ |
| Assay buffer | ✅ |
| HRP substrate | ✅ |
| Stop solution | ✅ |
| Wash buffer | ✅ |
| Plate covers | ✅ |
Everything you need — no additional reagents to purchase[reference:79].
Advantages
1. Excellent Sensitivity
With a limit of detection of 4 pg/mL and a calibration range of 7.8–500 pg/mL, this kit covers both normal and elevated IL-1β levels with outstanding sensitivity[reference:80].
2. High Specificity
No significant cross-reactivity or interference between human IL-1β and analogues has been observed[reference:81]. Your IL-1β signal is clean and specific.
3. Broad Sample Compatibility
Validated for serum, plasma, cell culture supernatants, and other biological fluids[reference:82] — versatile for diverse experimental setups.
4. Published Validation
This kit has been cited in 5 publications[reference:83] — providing real-world confidence from the research community.
5. EliKine™ Streptavidin-HRP Technology
The proprietary EliKine™ Streptavidin-HRP conjugates provide enhanced signal amplification compared to traditional HRP detection systems[reference:84].
6. Complete Kit
All components included — no hidden costs or last-minute reagent sourcing[reference:85].
7. Reliable Storage and Shipping
- Unopened kit: store at 2–8°C[reference:86]
- Shipped with gel pack and blue ice[reference:87]
Disadvantages
1. Human-Specific
This kit is specific to human IL-1β — not validated for mouse, rat, or other species[reference:88].
2. Research Use Only
Not intended for human or clinical diagnosis[reference:89].
3. Assay Duration
Standard sandwich ELISA with a working time of 3–5 hours[reference:90].
Who Should Buy This Kit?
✅ Buy this kit if you:
- Study inflammation, immunology, or autoimmune diseases
- Research infectious diseases and host-pathogen interactions
- Investigate cancer biology (tumor microenvironment, inflammation)
- Need to quantify human IL-1β in serum, plasma, or cell culture
- Require high sensitivity (4 pg/mL LOD)
- Value specificity — no cross-reactivity with IL-1α or other interleukins
- Want a complete, ready-to-use kit
- Appreciate EliKine™ Streptavidin-HRP amplification for better signal
- Prefer a kit with published validation (5 citations)
❌ Consider alternatives if you:
- Need to detect mouse or rat IL-1β (check Abbkine's other offerings)
- Are working with clinical diagnostic samples
- Require a faster assay (<3 hours)
Quick Specs Summary
| Parameter | Detail |
|---|---|
| Reactivity | Human |
| Target | Interleukin 1β (IL-1β) |
| Assay type | Sandwich ELISA (quantitative) |
| Detection method | Colorimetric (EliKine™ Streptavidin-HRP) |
| Calibration range | 7.8–500 pg/mL |
| Limit of detection | 4 pg/mL |
| Sample types | Serum, plasma, cell culture supernatants, biological fluids |
| Assay duration | 3–5 hours |
| Publications | 5 citations |
| Storage | 2–8°C (unopened) |
| Shipping | Gel pack with blue ice |
📌 Reference Link: EliKine™ Human IL-1β ELISA Kit — KTE6013