Human DACT2 ELISA Kit — Application Scenarios for Wnt Signaling, EMT, and Epigenetic Cancer Research

DACT2: The 83-kDa Methylation Sentinel at 6q27

DACT2 (Dapper Homolog 2 / DPr2 / DAPPER2) is an ~83 kDa cytoplasmic scaffold protein (UniProt: Q5SW24, Gene ID: 168002, Chr 6q27) that physically couples Dishevelled (Dvl) to β-catenin destruction dynamics, promotes lysosomal clearance of Nodal/TGF-β type I receptors, and — most clinically relevant — is frequently silenced by promoter CpG hypermethylation in ~50–90% of tumors across colon, breast, HCC, NPC, lung, gastric, and esophageal contexts[reference:12].

If your lab works on Wnt/β-catenin, EMT, or methylation-driven tumor suppression, you've almost certainly written the sentence "DACT2 was downregulated in our cancer cohort" — usually inferred from a qPCR melt curve or a methylation-specific PCR gel, and almost never backed by an actual protein-level measurement[reference:13]. That's a problem, because DACT2 is not just another "Wnt inhibitor" on a pathway diagram — it's the cytoplasmic scaffold/traffic-control protein that keeps β-catenin in the cytoplasm, maintains E-cadherin–β-catenin junctional localization, and restrains mesenchymal character.

Abbkine's Human Dapper homolog 2 (DACT2) ELISA Kit (KTE62149) is built to close that evidence gap: a two-site sandwich ELISA that captures this ~83 kDa cytoplasmic protein and turns it into a plate-readable concentration (ng/mL), so your epigenetic, EMT, or Wnt-inhibitor-rescue paper rests on a protein number — not a bisulfite band.

Cancer Research: DACT2 as a Methylated Tumor Suppressor

DACT2 is frequently silenced by promoter CpG hypermethylation in multiple cancers, and its loss correlates with poor survival. Oncology researchers use this kit to:

• Quantify DACT2 in tumor tissue lysates and patient serum/plasma
• Study DACT2 promoter methylation and protein-level silencing in cancer cohorts
• Investigate DACT2 as a prognostic biomarker in colon, breast, lung, and gastric cancers
• Monitor DACT2 re-expression in response to epigenetic therapies (DNMT inhibitors, HDAC inhibitors)

EMT and Metastasis Research

DACT2 silencing disrupts its regulation of epithelial-to-mesenchymal transition (EMT) and cytoskeleton reorganization. EMT researchers use this kit to:

• Quantify DACT2 in EMT models (TGF-β-induced, hypoxia-induced)
• Study DACT2-mediated β-catenin regulation and E-cadherin junctional localization
• Investigate DACT2's role in cell migration and invasion
• Monitor DACT2 levels during TGF-β/Nodal signaling manipulation

Wnt Signaling and Developmental Biology

DACT2 is a key modulator of Wnt/β-catenin signaling — it binds Dvl and β-catenin, promoting β-catenin phosphorylation/destruction and preventing nuclear translocation. Wnt researchers use this kit to:

• Quantify DACT2 in Wnt-active cell models and organoid cultures
• Study DACT2–Dvl–β-catenin complex dynamics
• Investigate DACT2 in embryonic development and tissue homeostasis
• Monitor DACT2 during stem cell differentiation

Drug Development: Pharmacodynamic Biomarker for Epigenetic Therapies

As epigenetic therapies targeting DACT2 re-expression enter preclinical pipelines, there's growing demand for pharmacodynamic biomarkers. Drug developers use this kit to:

• Monitor DACT2 re-expression in response to DNMT inhibitors
• Assess target engagement in epigenetic therapy trials
• Study DACT2 as a surrogate readout for Wnt pathway inhibition
• Investigate DACT2 rescue as a therapeutic strategy

Why Choose This Kit for Your DACT2 Research?

Kit Components at a Glance

• Human DACT2 microplate (pre-coated with capture antibody)
• Human DACT2 standard
• Human DACT2 detection antibody (biotin-conjugated)
• Streptavidin-HRP
• Standard diluent, assay buffer, HRP substrate, stop solution, wash buffer, plate covers

🔬 Which research field does your work belong to?
Cancer Research | EMT & Metastasis | Wnt Signaling | Epigenetic Therapy | Other
Share your application with us — we'd love to hear how you're using this kit in your studies!

📌 Reference Link: Human Dapper homolog 2 (DACT2) ELISA Kit — KTE62149