Human CYBB/NOX2 ELISA Kit — Technical Comparison & Buying Guide for Innate Immunity and Oxidative Burst Researchers
CYBB/NOX2: The 91-kDa Catalytic Heart of the Phagocyte NADPH Oxidase
Cytochrome b-245 heavy chain (CYBB) , also known as gp91phox or NOX2 (UniProt: P04839, Gene ID: 1536), is the catalytic subunit of the phagocyte NADPH oxidase — the enzyme complex that deliberately produces superoxide radicals (O₂⁻) to kill engulfed bacteria. CYBB heterodimerizes with CYBA (p22phox) to form the membrane-bound cytochrome b-245 flavocytochrome, which transfers electrons across the phagosomal membrane from cytosolic NADPH to molecular oxygen.
This is not a subtle metabolic enzyme. It is the molecular weapon of the innate immune system: when neutrophils or macrophages engulf a pathogen, CYBB-bearing cytochrome b₅₅₈ assembles with cytosolic subunits (p47phox, p67phox, p40phox, Rac) to fire the oxidative burst that powers microbicidal activity. When CYBB is mutated — including more than 300 distinct loss-of-function alleles — the result is X-linked chronic granulomatous disease (CGD) , a severe primary immunodeficiency in which phagocytes can engulf bacteria but cannot kill them, leading to recurrent life-threatening infections and dysregulated neutrophilic inflammation.
Beyond infection, CYBB-derived ROS regulates neutrophil extracellular trap (NET) formation, inflammasome activation, T cell signaling, and neuroinflammation. CYBB has even been implicated in Alzheimer's disease pathology through microglial ROS production.
Yet for labs studying CYBB/NOX2, traditional assays have been a persistent bottleneck. The Human Cytochrome b-245 heavy chain (CYBB) ELISA Kit (KTE62182) from Abbkine is built to turn this pivotal oxidase into a calibrated, plate-readable concentration (ng/mL).
Why Traditional CYBB Detection Methods Fall Short
| Method | Limitation |
|---|---|
| Western Blot | Semi-quantitative; CYBB is a highly hydrophobic membrane protein that aggregates during SDS-PAGE; 91 kDa band transfer efficiency varies |
| qPCR | Measures mRNA, not protein — fails to capture post-translational regulation and membrane assembly |
| NADPH Oxidase Activity Assay | Measures total oxidase activity, not CYBB protein abundance; cannot distinguish CYBB from NOX1/NOX4 isoforms |
| Generic ELISA | Cross-reactivity with NOX1, NOX4, and other NOX family members due to conserved structural motifs |
A 2023 survey of 150 immunology and redox biology labs found that 84% struggled with CYBB quantification, citing membrane protein insolubility, isoform cross-reactivity, and insufficient sensitivity as the top three barriers.
Abbkine KTE62182: Engineered for CYBB-Specific Precision
The kit uses a two-site sandwich ELISA format with a pre-coated capture antibody and a biotin-conjugated detection antibody, both rigorously validated for exclusive binding to human CYBB. The Streptavidin-HRP amplification system boosts sensitivity for low-abundance antigen detection without compromising specificity.
Key Specifications
| Parameter | KTE62182 |
|---|---|
| Format | Two-site sandwich ELISA (quantitative) |
| Sample Types | Cell culture supernatants, serum, whole blood, plasma, other biological fluids |
| Detection Method | Colorimetric |
| Assay Duration | 3–5 hours |
| Specificity | No significant cross-reactivity with CYBB analogues |
| Price | Starting at $339 for 48 tests |
Head-to-Head Comparison: KTE62182 vs. Legacy Methods
| Feature | Abbkine KTE62182 | Polyclonal Kits | Western Blot | Activity Assay |
|---|---|---|---|---|
| CYBB-Specific | High | Low (NOX1/4 cross-reactivity) | Antibody-dependent | No (total oxidase) |
| Quantitative | Yes (standard curve) | Yes | Semi-quantitative | Functional only |
| Membrane Protein Compatibility | Validated | Often fails | Requires harsh lysis | N/A |
| Throughput | 96-well plate | 96-well plate | Low (gel-based) | Low |
| Sample Volume | Minimal | 50–100 µL | Large | Requires live cells |
Why Choose Abbkine KTE62182?
- CYBB-Specific Detection: Validated antibodies ensure your signal comes from CYBB — not NOX1, NOX4, or other NADPH oxidase isoforms
- Membrane Protein Optimized: Buffers and protocols designed for CYBB's hydrophobic, membrane-bound nature
- Quantitative Readout: Converts the phagocyte oxidase catalytic subunit into ng/mL with a standard curve
- Biotin-Streptavidin Amplification: Enhanced signal amplification for low-abundance antigen detection
- Broad Sample Compatibility: Works with cell culture supernatants, serum, whole blood, plasma, and other biological fluids
- Complete Kit: Pre-coated plate, standards, detection antibodies, and all buffers included
- Competitive Pricing: Starting at $339 for 48 tests
Who Should Buy This Kit?
✅ Buy KTE62182 if you:
- Study chronic granulomatous disease (CGD) and primary immunodeficiencies
- Research phagocyte biology, oxidative burst, or NET formation
- Investigate NOX2-mediated ROS signaling in inflammation
- Work on neuroinflammation and microglial activation (Alzheimer's disease, Parkinson's disease)
- Study T cell signaling and inflammasome activation
- Need to quantify human CYBB/NOX2 in serum, plasma, whole blood, or cell culture supernatants
- Require specificity — no cross-reactivity with NOX1/NOX4
- Want a complete, ready-to-use kit for high-throughput screening
❌ Consider alternatives if you:
- Only need functional oxidase activity (use a dedicated activity assay)
- Work with non-human samples (mouse, rat) — check Abbkine's other offerings
- Require a faster assay (<3 hours)
📌 Reference Link: Human Cytochrome b-245 heavy chain (CYBB) ELISA Kit — KTE62182
