| Product name | Human Dapper homolog 2 (DACT2) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human Dapper homolog 2 (DACT2) ELISA Kit employs a two-site sandwich ELISA to quantitate DACT2 in samples. An antibody specific for DACT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDACT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DACT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DACT2 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | DACT2; C6orf116; DAPPER2; DPR2; FLJ31232; MGC133141; MGC133142; RP11-503C24.7; bA503C24.7; dapper homolog 2; antagonist of beta-catenin; dapper; antagonist of beta-catenin; homolog 2 |
| Kit components | • Human Dapper homolog 2 microplate • Human Dapper homolog 2 standard • Human Dapper homolog 2 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human Dapper homolog 2 (DACT2) ELISA Kit has high sensitivity and excellent specificity for detection of Human DACT2. No significant cross-reactivity or interference between Human DACT2 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | DACT2 (Dishevelled Binding Antagonist Of Beta Catenin 2) is a Protein Coding gene. Among its related pathways are TGF-beta receptor signaling. GO annotations related to DACT2 include transcription factor binding and protein kinase C binding. An important paralog of DACT2 is DACT1. Involved in regulation of intracellular signaling pathways during development. Negatively regulates the Nodal signaling pathway, possibly by promoting the lysosomal degradation of Nodal receptors, such as TGFBR1. May be involved in control of the morphogenetic behavior of kidney ureteric bud cells by keeping cells epithelial and restraining their mesenchymal character. May play an inhibitory role in the re-epithelialization of skin wounds by attenuating TGF-beta signaling (By similarity). |
| Gene ID | 168002 |
| Alternative | DACT2; C6orf116; DAPPER2; DPR2; FLJ31232; MGC133141; MGC133142; RP11-503C24.7; bA503C24.7; dapper homolog 2; antagonist of beta-catenin; dapper; antagonist of beta-catenin; homolog 2 |
| Accession | Q5SW24 |
Fig.1. Human Dapper homolog 2 (DACT2) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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