CheKine™ Micro MPO Activity Assay Kit: The Gold Standard for Inflammation and Oxidative Stress Research
MPO: The Master Marker of Neutrophil Activation
Myeloperoxidase (MPO) is a leukocyte enzyme secreted by activated neutrophils and mononuclear macrophages, mainly stored in the azurophilic granules of these immune cells.[reference:0] When white blood cells are activated, MPO is released into phagocytic vesicles and plasma, inducing oxidative stress and tissue damage under specific conditions—making it a critical biomarker of systemic inflammation and oxidative stress.[reference:1]
Accurate MPO activity measurement is essential for understanding inflammatory diseases, cardiovascular pathology, and neutrophil-mediated tissue injury.
Why Abbkine KTB1152 Leads the Field
| Feature | Abbkine KTB1152 | Traditional MPO Assays |
|---|---|---|
| Detection Principle | H₂O₂ decomposition + o-dianisidine oxidation (460 nm)[reference:2] | Variable; often complex |
| Sample Types | Animal tissues, cells, plasma, serum, other liquids[reference:3] | Limited compatibility |
| Sensitivity | Colorimetric—linear correlation with MPO activity[reference:4] | Variable |
| Protocol | Easy to operate, verified samples[reference:5] | Often cumbersome |
| Storage | 6 months at -20°C, protected from light[reference:6] | Shorter shelf life |
| Publications | 0 (reflects novelty—be the first to cite!)[reference:7] | Varies |
The o-Dianisidine Oxidation Principle
In the kit, MPO catalyzes the decomposition of H₂O₂ while simultaneously oxidizing o-dianisidine to produce colored substances. The reaction product exhibits a characteristic absorption peak at 460 nm, with color depth linearly proportional to MPO activity.[reference:8] This simple colorimetric readout eliminates the need for specialized instrumentation.
Applications Across Research Fields
- Cardiovascular Research — MPO is a strong predictor of coronary artery disease and heart failure
- Inflammatory Diseases — tracking neutrophil activation in rheumatoid arthritis, IBD, and sepsis
- Cancer Immunology — understanding tumor-associated neutrophil biology
- Drug Discovery — screening anti-inflammatory compounds and MPO inhibitors
Critical Sample Handling Tips
- If not assayed immediately, samples can be stored at -80°C for one month[reference:9]
- Fresh samples are necessary for optimal results[reference:10]
- It is recommended to perform several dilutions to ensure readings fall within the standard value range[reference:11]
- Complete sample preparation before storing if not assaying immediately[reference:12]
The Bottom Line
For researchers investigating inflammation, oxidative stress, or neutrophil biology, KTB1152 delivers a simple, reliable, and cost-effective solution for MPO activity quantification. With no publications yet—yours could be the first to showcase this kit's capabilities.
👉 Measure MPO activity with confidence. View Product
