How to analyze the fluorescence intensity of cells by using Image J?
Today we're going to talk about Image J’s use in immunohistochemistry. Image J software is completely free and easy to download. As a scientific researcher, we face all kinds of maddening experimental data every day. If we can skillfully use Image J, it will undoubtedly be very helpful for us to analyze the experimental results. Now let's introduce how to analyze immunohistochemistry by Image J. Here is an example: Step 1: Import Image: Open Image J, File→Open: Open the Image to be measured Step 2: Transform the Image format: Image→Type→RGB Stack At this time, a scroll bar appears at the bottom of the picture. Files 1, 2, and 3 correspond to red, green, and blue respectively. Select relatively clear pictures…
Alkaline phosphatase and Bone Metabolism
AKP / ALP is a zinc-containing glycoprotease that can hydrolyze various natural and synthetic phospholipid monoester compounds in an alkaline environment. AKP / ALP is widely distributed in various organs of the human body, mainly the liver. An alkaline phosphatase level test (ALP test) measures the amount of alkaline phosphatase enzyme in your bloodstream. The test requires a simple blood draw and is often a routine part of other blood tests. An ALP test may be performed to determine how well your liver and gallbladder are functioning or to identify problems with your bones. Figure 1: Smart nanoprobes in the detection of ALP(Picture Source: https://pubs.rsc.org ) Bone and liver alkaline phosphatases are the predominant alkaline phosphatase (ALP) isoenzymes in serum…
Abbkine mitochondrial respiratory chain complex series detection kits and tricarboxylic acid cycle series detection kits
Some time ago, Abbkine launched a lot of rare treasures with full horsepower, and quickly captured the hearts of a large number of heroes and knights on the rivers and lakes, and received rave reviews. To this end, the editor deliberately shows the members who have recently debuted in the center of the traffic. Type Item No. Product Name Flow Explosion Series BMU101-EN SuperKine™ West Pico PLUS Chemiluminescent Substrate BMU102-EN SuperKine™ West Femto Maximum Sensitivity Substrate BMU103-EN SuperKine™ Enhanced Antibody Dilution Buffer KTD101-EN Universal Loading Control Antibody Cocktail Mitochondrial respiratory chain complex series KTB1850 CheKine™ Mitochondrial complex I Activity Assay Kit (colorimetric) KTB1860 CheKine™ Mitochondrial complexⅡ Activity Assay Kit (colorimetric) KTB1870 CheKine™ Mitochondrial complex Ⅲ Activity Assay Kit (colorimetric) KTB1880…
Promotion of the cell status detection kits
Do you have any trouble in the process of exploring the cell's proliferation, aging, and apoptosis? Perhaps there are some concerns about that weak fluorescence will interfere with your judgment? Or did the cumbersome steps block your thoughts? Annexin V of AbFluor™ - Increases Fluorescence Intenacity by 30% or More! It saves half of the operation time with the EDU Kit! During this summer, the Abbkine's cell state assay kits will "witness" the vitality, apoptosis and aging in the world of cells with you! Promotion participants: Authorized Distributors Overseas. Promotion time: From now to 30th September, 2021. Promotion products: Cell status detection in the attached list (Table 1). Promotion Discount: 55% off. Table 1: Product Name Cat. No…
Universal and Stable SuperKine™ Enhanced Antibody Dilution Buffer
It is a ready-to-use universal antibody diluent suitable for WB, IF, IHC, ELISA and other immunological detection experiments. The enhancer, blocking agent and stabilizer of the antibody binding reaction are added to enhance the immune response, reduce non-specific binding. It can be used repeatedly and save valuable antibodies. This product does not contain protein,w phosphate, sodium azide or thimerosal preservatives, and is suitable for dilution of most types of antibodies, including HRP and AP-labeled antibodies.
SuperKine™ ECL substrate with High sensitivity and durability
The Western Blot HRP substrate series products are composed of peroxide reagent and luminol/enhancer, which are mixed and incubated with the blotting membrane, then film or imaging system can be used to detect the results. SuperKine™ enhanced/hypersensitive ECL substrates are based on the innovation and optimization of the classic peroxidase substrate system. Because of higher sensitivity and better stability, Abbkine ECL series products can save your expensive antibodies, efficiently present low-abundance protein expression, and reduce non-specific band interference. Even using after half of a year, it can still have a stable and ultrasensitive performance. It is the best choice with excellent quality and affordable prices to meet your various experimental needs.
Getting more research benefits from innovative Cell Counting Kit-8 with less cost
Abbkine Cell counting kit-8 is a colorimetric assay kit for cell number counting, cell prolifiration and cytotoxicity assays. By adding CCK-8 directly to the cell media, CCK-8 kit shows fast, high-throughput screening without a solubilization process, obtaining highly reproducible and accurate results.
Featured IPKine™ secondary antibody for Western Blot after Immunoprecipitation
25 kD or 50 kD protein detection on Western blots after Immunoprecipitation is often suffered from heavy or light chain blotting contamination. Abbkine’s IPKine™ products could solve these problems and bring you satisfying results with good performance.
Dedicated IFKine™ antibodies for multiple immunofluorescence labelling
Abbkine IFKine™ is a series of specially optimized secondary antibodies with improved brightness, photostability and less nonspecific hybridization and background. Donkey host and other species of serum/IgG absorbed make it ideal choice for fluorescence staining, especially in fluorescence multiple labelling.
Easy-to-use DyLight® conjugated secondary antibodies
DyLight fluorescent dyes are a new family of dyes with improved brightness and photostability. Abbkine offers comprehensive portfolio of DyLight® conjugated secondary antibodies with high specific and multiple applications to meet and satisfy your most types of immunoassay.
