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【New product focus】Abbkine mitochondrial respiratory chain complexs ( I~V ) detection kits

Date:2021-05-21 Views:203

Following the launch of the new mitochondrial respiratory chain complex detection kit in the previous issue, many fans are very interested in several products of this series with high cost performance. It is expected that I will talk about these products today !

Background

The mitochondrial respiratory chain is mainly composed of mitochondrial respiratory chain enzymes. Mitochondrial respiratory chain complex I (NADH oxidase) and mitochondrial respiratory chain complex II (succinate dehydrogenase) are the main elements for electrons to enter the mitochondrial electron transport chain (ETC). Complex I catalyzes the oxidation of NADH, and complex II catalyzes the oxidation of succinic acid to fumaric acid. Subsequently, coenzyme Q (Q) forms coenzyme (QH2), which eventually leads to a decrease in the terminal electron acceptor O2. Mitochondrial respiratory chain complex III (cytochrome c oxidoreductase) is an essential protein for mitochondrial oxidative phosphorylation. Mitochondrial respiratory chain complex III is the gatekeeper of the mitochondrial respiratory chain and the third main source of reactive oxygen species. Mitochondrial respiratory chain complex IV (cytochrome c oxidase) is the terminal electron acceptor of the mitochondrial electron transport chain. Complex IV converts O2 into water through the oxidation of cytochrome c. This process is related to mitochondrial cell membrane ATP synthesis. Mitochondrial respiratory chain complex V and the above four complexes complete oxidative phosphorylation to generate ATP, which is called ATP synthase, also called F1F0-ATPase.

Respiratory chain composition

The respiratory chain for respiration is composed of five protein complexes with relatively independent functions, namely complex I-V. Strictly speaking, the respiratory chain only contains complexes I to IV, and ATP synthase does not belong to the respiratory chain, but from the perspective of a complete ATP synthesis chain, ATP synthase is generally also called complex V.

ATP synthesis chain

 

Respiratory chain function

The mitochondrial respiratory chain is mainly related to energy metabolism and oxidative phosphorylation. The functions of its five components (the former: complex I~IV, the latter complex V) are as follows:

The former-the electron transport chain, oxidizes NADH and FADH2 produced by glycolysis and tricarboxylic acid cycle, while pumping protons out of the cell membrane or mitochondrial inner membrane to generate a proton gradient;

The latter-ATP synthase, also known as complex V, uses the influx of protons caused by the proton gradient to synthesize ADP and phosphoric acid into ATP, thereby storing the energy generated by hydrogen carrier oxidation in the form of ATP.

The mitochondrial respiratory chain

 

Assay principle& Sample type

Catalog No Product Name Assay principle Sample type
KTB1850 CheKine™ Mitochondrial complex I Activity

Assay Kit (colorimetric)

Complex I can catalyze the dehydrogenation of NADH to NAD+. The oxidation rate of NADH can be determined at 340 nm to calculate the activity of Complex I. Animal/plant tissue and cell samples.
KTB1860 CheKine™ Mitochondrial complexⅡ Activity

Assay Kit (colorimetric)

The reduced coenzyme Q, which is the catalytic product of the mitochondrial respiratory chain complex II, can further reduce 2,6-dichloroindoxyl, which has a characteristic absorption peak at 605 nm. So, the activity of complex II could be calculated according to the decrease rate of 2,6-dichloroindoxyl. Animal/plant tissue and cell samples.
KTB1870 CheKine™ Mitochondrial complex Ⅲ Activity

Assay Kit (colorimetric)

Complex III could transfer the hydrogen from reduced CoQ to cytochrome C to produce reduced cytochrome C. Unlike oxidized cytochrome C, reduced cytochrome C has characteristic light absorption at 550 nm. So, the increasing of light absorption rate can reflect the activity of Complex III enzymes. Animal/plant tissue and cell samples.
KTB1880 CheKine™ Mitochondrial complex Ⅳ Activity

Assay Kit (colorimetric)

Reduced cytochrome C has characteristic light absorption at 550nm. Mitochondrial respiratory chain complex IV could catalyze reduced cytochrome C to oxidized cytochrome C. Therefore, the decreasing rate of light absorption at 550nm can reflect the enzyme activity of mitochondrial respiratory chain complex IV. Animal/plant tissue and cell samples.
KTB1890 CheKine™ Mitochondrial complex Ⅴ Activity Assay Kit (colorimetric) Complex Ⅴ hydrolyzes ATP to produce ADP and Pi. The activity of complex Ⅴ is determined by measuring Pi increase rate. Animal/plant tissue and cell samples.

 

Features & Benefits

  • Simple procedure; Fast and convenient.
  • All the required reagents and standards for experiment are provided .
  • The detailed sample preparation methods are provided .
  • The detailed calculation method and standard curve are provided for reference.
  • The results are more stable and reliable as it is verified in many different types of samples.
  • A suitable method for determination of protein concentration for each kit is provided.

 

Data Analysis

Data Analysis

Data Analysis

Data Analysis

 

Related Products

Catalog No Product Name Size
KTB1850 CheKine™ Mitochondrial complex I Activity

Assay Kit (colorimetric)

48T/96T
KTB1860 CheKine™ Mitochondrial complexⅡ Activity

Assay Kit (colorimetric)

48T/96T
KTB1870 CheKine™ Mitochondrial complex Ⅲ Activity

Assay Kit (colorimetric)

48T/96T
KTB1880 CheKine™ Mitochondrial complex Ⅳ Activity

Assay Kit (colorimetric)

48T/96T
KTB1890 CheKine™ Mitochondrial complex Ⅴ Activity

Assay Kit (colorimetric)

48T/96T
KTB1023 CheKine™ Citrate Synthase (CS) Activity

Assay Kit( Colorimetric)

48T/96T
KTB1230 CheKine™ Succinate Dehydrogenase(SDH) Activity

Assay Kit (Colorimetric)

96T
KTB1270 CheKine™ Pyruvate Dehydrogenase (PDH) Activity  Assay Kit (Colorimetric) 48T/96T
KTB1240 CheKine™ α-Ketoglutarate Dehydrogenase (α-KGDH) Assay Kit  (Colorimetric) 48T/96T
KTB1260 CheKine™ Acetyl Coenzyme A (Acetyl-CoA)

Assay Kit (Colorimetric)

48T/96T
KTB1250 CheKine™ Mitochondrial Isocitrate Dehydrogenase(ICDHm)Assay Kit (Colorimetric ) 48T/96T

 

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