A23010 | DyLight 350, Goat Anti-Mouse IgG — The UV-Excitation Fluorophore That Opens Up New Multiplexing Possibilities

DyLight 350: UV-Excitation Fluorescence for Multiplexing Mastery

When you're building a multiplex panel, every channel counts. The blue/violet region (excitation ~350 nm, emission ~440 nm) is often underutilized—but it's a valuable channel for nuclear counterstaining, cellular tracing, or adding an extra parameter to your flow cytometry panel. Abbkine's DyLight 350, Goat Anti-Mouse IgG (A23010) gives you access to this UV-excitable channel with the brightness and photostability that DyLight dyes are famous for.

Why DyLight 350 Outperforms Traditional UV Dyes

DyLight fluorescent dyes are a new family of dyes with improved brightness and photostability [5†L11-L12]. DyLight 350-antibody conjugates are highly water-soluble and remain fluorescent from pH 4 to pH 9 [5†L19-L20]. The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody—all optimized by Abbkine for each conjugate [5†L13-L19].

Affinity Purified, DyLight 350-Conjugated, Mouse-Specific

A23010 is an affinity-purified polyclonal antibody raised in goat against mouse IgG whole molecule [5†L4-L5]. It reacts with the heavy chains of mouse IgG and the light chains of all other mouse immunoglobulins, with no reactivity on non-immunoglobulin serum proteins [5†L37-L39]. The DyLight 350 conjugate provides bright, photostable fluorescence for imaging and flow cytometry applications.

Applications and Recommended Dilutions

Validated for fluorescent applications with suggested starting dilutions of 1:50–1:1,000 for most fluorescent applications [5†L5-L8]:

• Flow Cytometry (FCM)
• Immunocytochemistry (ICC)
• Immunofluorescence (IF)

Product Specifications

• Host: Goat
• Reactivity: Mouse
• Clonality: Polyclonal
• Purity: >95% based on SDS-PAGE (affinity purified using solid-phase mouse IgG)
• Conjugate: DyLight 350
• Formulation: Liquid in PBS, pH 7.4, with 0.02% sodium azide, 1% BSA, and 50% glycerol [5†L20-L22]
• Stability: One year at -20°C from shipment [5†L22-L23]

3 Publications — and Growing

A23010 has been cited in 3 publications [5†L3]. As multiplexing becomes increasingly central to modern research, the demand for UV-channel reagents continues to grow.

Who Needs This Antibody?

• Flow cytometrists building multi-color panels
• Cell biologists performing multiplex immunofluorescence
• Pathologists needing UV-excitable nuclear or cellular markers
• Any researcher wanting to add a blue/violet channel to their detection arsenal

🔗 Reference Link: https://www.abbkine.com/product/dylight-350-goat-anti-mouse-igg-a23010/