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TraKine™ F-actin Staining Kit (Green Fluorescence)

Product Information

  • Product name

    TraKine™ F-actin Staining Kit (Green Fluorescence)

  • Application notes

    Abbkine TraKine™ F-actin Staining Kit (Green Fluorescence) uses a green fluorescent (Ex/Em=490/515nm) phalloidin conjugate selectively bound to F-actins, it is much higher photostability than the other fluorescein-phalloidin conjugates. Phalloidin derivatives are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, which can stain F-actins in cell cultures or cell-free experiments at nanomolar levels.

Product Properties

  • Kit components

    • Phalloidin (Green Fluorescence) (200×)
    • Assay Buffer (5×)

  • Features & Benefits

    • Optimized staining protocol for labeling, identifying and quantitating F-actin in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments.
    • Proprietary Phalloidin (Green Fluorescence) (Ex/Em = 490/515 nm)-high-affinity probe for F-actins and much higher photostability than the other fluorescein-phalloidin conjugates.

  • Usage notes

    As the optimal staining conditions may vary among different cell types, we recommend that a suitable concentration of Phalloidin (Green Fluorescence) be determined.

  • Storage instructions

    Refer to list of materials supplied for storage conditions of individual components. Stable for at least 6 months at recommended temperature from date of shipment.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Phalloidin belongs to a class of toxins called phallotoxins, which are isolated from the death cap mushroom (Amanita phalloides). It is a bicyclic peptide that binds to F-actin specifically. Therefore, the distribution of F-actin can be very conveniently studied by using a fluorescent dye-labeled phalloidin. Inside the phalloidin, there is an unusual thioether bridge between cysteine and tryptophan, which can form an inner ring structure. When the pH is raised, the thioether is cleaved and the phalloidin loses its affinity for actin.

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TraKine™ F-actin Staining Kit (Green Fluorescence)
Click to download booklet for more details

Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact for your enquiry.

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through if you need more information or support.

Cat #: KTC4008

  • Size
  • Price
  • 50T $50
  • 300T $160
  • 1000T $420

Contact information

Do not hesitate to contact us if you have any questions.

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  •  Fax: +86-27-59716788
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