| Product name | TraKine™ F-actin Staining Kit (Green Fluorescence) |
| Applications notes | Abbkine TraKine™ F-actin Staining Kit (Green Fluorescence) uses a green fluorescent (Ex/Em=490/515nm) phalloidin conjugate selectively bound to F-actins, it is much higher photostability than the other fluorescein-phalloidin conjugates. Phalloidin derivatives are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, which can stain F-actins in cell cultures or cell-free experiments at nanomolar levels. |
| Kit components | • Phalloidin (Green Fluorescence) (200×) • Assay Buffer (5×) |
| Features & Benefits | • Optimized staining protocol for labeling, identifying and quantitating F-actin in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. • Proprietary Phalloidin (Green Fluorescence) (Ex/Em = 490/515 nm)-high-affinity probe for F-actins and much higher photostability than the other fluorescein-phalloidin conjugates. |
| Usage notes | As the optimal staining conditions may vary among different cell types, we recommend that a suitable concentration of Phalloidin (Green Fluorescence) be determined. |
| Storage instructions | Refer to list of materials supplied for storage conditions of individual components. Stable for at least 6 months at recommended temperature from date of shipment. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Phalloidin belongs to a class of toxins called phallotoxins, which are isolated from the death cap mushroom (Amanita phalloides). It is a bicyclic peptide that binds to F-actin specifically. Therefore, the distribution of F-actin can be very conveniently studied by using a fluorescent dye-labeled phalloidin. Inside the phalloidin, there is an unusual thioether bridge between cysteine and tryptophan, which can form an inner ring structure. When the pH is raised, the thioether is cleaved and the phalloidin loses its affinity for actin. |
Author：Wang, Wenzhao, et al. Publication name：Composites Part B: Engineering IF：13.1
Author： Hong Y, Xu WQ, Feng J Publication name：ACTA PHARMACOLOGICA SINICA IF：7.189
Author：Q Ma, G Wang, N Li, X Wang, X Kang, Y Mao Publication name：Experimental Cell Research IF：3.7
Author：Z Zhang, W Wu, H Jiao, Y Chen, X Ji Publication name：British Journal of Pharmacology IF：3.4
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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