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Mouse Transient receptor potential cation channel subfamily V member 2 (TRPV2) ELISA Kit

Mouse Transient receptor potential cation channel subfamily V member 2 (TRPV2) ELISA Kit

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Specification

Product name Mouse Transient receptor potential cation channel subfamily V member 2 (TRPV2) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Transient receptor potential cation channel subfamily V member 2 (TRPV2) ELISA Kit employs a two-site sandwich ELISA to quantitate TRPV2 in samples. An antibody specific for TRPV2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRPV2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRPV2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRPV2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TRPV2; MGC12549; VRL; VRL-1; VRL1; vanilloid receptor-like protein 1

Product Properties

Kit components • Mouse Transient receptor potential cation channel subfamily V member 2 microplate
• Mouse Transient receptor potential cation channel subfamily V member 2 standard
• Mouse Transient receptor potential cation channel subfamily V member 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Transient receptor potential cation channel subfamily V member 2 (TRPV2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TRPV2. No significant cross-reactivity or interference between Mouse TRPV2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TRPV2 encodes an ion channel that is activated by high temperatures above 52 °C. The protein may be involved in transduction of high-temperature heat responses in sensory ganglia. It is thought that in other tissues the channel may be activated by stimuli other than heat.TRPV2 is activated by high temperatures above 52 °C. Alternatively it can be activated at lower temperatures by chemicals, such as the research tool 2-APB, the plant cannabinoid cannabidiol, and probenecid. It is blocked by ruthenium red and lanthanum. TRPV2 was independently discovered by two research groups and described in 1999. It was identified in the lab of David Julius as a close homolog of TRPV1, the first identified thermosensitive ion channel.
Gene ID 22368
Alternative TRPV2; MGC12549; VRL; VRL-1; VRL1; vanilloid receptor-like protein 1
Accession Q9WTR1

Image & description

Fig.1. Mouse Transient receptor potential cation channel subfamily V member 2 (TRPV2) Standard Curve.

Fig.1. Mouse Transient receptor potential cation channel subfamily V member 2 (TRPV2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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