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RFP-Tag Polyclonal Antibody

RFP-Tag Polyclonal Antibody

Views(924) Publications(0) Catalog no(ABP50149)
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Specification

Product name RFP-Tag Polyclonal Antibody
Immunogen Recombinant Protein
Host Rabbit
Applications WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000).
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen
Alternative RFP; GFP like chromoprotein; red fluorescent protein; Red fluorescent protein eqFP611

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Red Fluorescent Protein (RFP) is isolated from sea anemone (Discosoma sp), and commonly used as a molecular tag to study protein localization, reporter gene expression, and protein-protein interactions. Although it exists as a homotetramer, monomeric variants have been constructed for use in various applications.
Alternative RFP; GFP like chromoprotein; red fluorescent protein; Red fluorescent protein eqFP611
Others The antibody detects RFP tag fusion proteins.

Image & description

Fig. 2ug RFP fusion protein+ Primary antibody dilution at 1) 1:5000, 2) 1:20000. Secondary antibody was diluted at 1:20000.

Fig. 2ug RFP fusion protein+ Primary antibody dilution at 1) 1:5000, 2) 1:20000. Secondary antibody was diluted at 1:20000.

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