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Human Alpha-2-antiplasmin (SERPINF2) ELISA Kit

Human Alpha-2-antiplasmin (SERPINF2) ELISA Kit

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Specification

Product name Human Alpha-2-antiplasmin (SERPINF2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Alpha-2-antiplasmin (SERPINF2) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINF2 in samples. An antibody specific for SERPINF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINF2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SERPINF2; A2AP; AAP; ALPHA-2-PI; API; PLI; OTTHUMP00000115656; alpha-2-antiplasmin; alpha-2-plasmin inhibitor; serine (or cysteine) proteinase inhibitor; clade F (alpha-2 antiplasmin; pigment epithelium derived

Product Properties

Kit components • Human Alpha-2-antiplasmin microplate
• Human Alpha-2-antiplasmin standard
• Human Alpha-2-antiplasmin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Alpha-2-antiplasmin (SERPINF2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SERPINF2. No significant cross-reactivity or interference between Human SERPINF2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background An inherited hemorrhagic diathesis due to plasmin inhibitor deficiency was described by Koie et al. (1978) in a 25-year-old Okinawa man. He had suffered prolonged bleeding and ecchymoses after minor trauma, spontaneous joint hemorrhage, and one episode of hemothorax. The bleeding episodes were reduced in frequency and severity by an antiplasminic drug. Laboratory abnormalities were limited to shortened euglobulin-lysis time and whole blood clot lysis time. No circulating alpha-2-plasmin inhibitor was found in the plasma. Even though only one case was observed, autosomal recessive inheritance was undoubted because the parents were consanguineous and both had plasma antiplasmin levels about half normal. The authors called the condition Miyasato disease after the proband's surname.
Alternative SERPINF2; A2AP; AAP; ALPHA-2-PI; API; PLI; OTTHUMP00000115656; alpha-2-antiplasmin; alpha-2-plasmin inhibitor; serine (or cysteine) proteinase inhibitor; clade F (alpha-2 antiplasmin; pigment epithelium derived

Image & description

Fig.1. Human Alpha-2-antiplasmin (SERPINF2) Standard Curve.

Fig.1. Human Alpha-2-antiplasmin (SERPINF2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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