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Rat Thromboxane A2 (TXA2) ELISA Kit

Rat Thromboxane A2 (TXA2) ELISA Kit

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Product name Rat Thromboxane A2 (TXA2) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Thromboxane A2 (TXA2) ELISA Kit employs a two-site sandwich ELISA to quantitate TXA2 in samples. An antibody specific for TXA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTXA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TXA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TXA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TXA2

Product Properties

Kit components • Rat Thromboxane A2 microplate
• Rat Thromboxane A2 standard
• Rat Thromboxane A2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Thromboxane A2 (TXA2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TXA2. No significant cross-reactivity or interference between Rat TXA2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Thromboxane B2 is a metabolite of thromboxane A2 which is known to be highly unstable under physiological conditions. Thromboxane B2 is recognized as a valuable substance for study of a variety of biochemical processes as well as important synthetic precursor to thromboxane A2.Several syntheses of thromboxane B2 have been completed, either in racemic form or deriving the stereocenters from a chiral pool. The Burke group is currently endeavoring to complete the total synthesis of thromboxane B2. Thromboxane B2 is an inactive metabolite/product of thromboxane A2. It is almost completely cleared in the urine.It itself is not involved in platelet activation and aggregation in case of a wound, but its precursor, thromboxane A2, is. Thromboxane A2 synthesis is the target of the drug aspirin, which inhibits the COX-1 enzyme (the source of thromboxane A2 in platelets).
Alternative TXA2

Image & description

Fig.1. Rat Thromboxane A2 (TXA2) Standard Curve.

Fig.1. Rat Thromboxane A2 (TXA2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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