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Mouse Tumor necrosis factor ligand superfamily member 9 (TNFSF9) ELISA Kit

Mouse Tumor necrosis factor ligand superfamily member 9 (TNFSF9) ELISA Kit

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Specification

Product name Mouse Tumor necrosis factor ligand superfamily member 9 (TNFSF9) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Tumor necrosis factor ligand superfamily member 9 (TNFSF9) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF9 in samples. An antibody specific for TNFSF9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFSF9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFSF9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFSF9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TNFSF9; 4-1BB-L; CD137L; homolog of mouse 4-1BB-L; receptor 4-1BB ligand

Product Properties

Kit components • Mouse Tumor necrosis factor ligand superfamily member 9 microplate
• Mouse Tumor necrosis factor ligand superfamily member 9 standard
• Mouse Tumor necrosis factor ligand superfamily member 9 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Tumor necrosis factor ligand superfamily member 9 (TNFSF9) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNFSF9. No significant cross-reactivity or interference between Mouse TNFSF9 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TNFSF9 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.
Gene ID 21950
Alternative TNFSF9; 4-1BB-L; CD137L; homolog of mouse 4-1BB-L; receptor 4-1BB ligand
Accession P41274

Image & description

Fig.1. Mouse Tumor necrosis factor ligand superfamily member 9 (TNFSF9) Standard Curve.

Fig.1. Mouse Tumor necrosis factor ligand superfamily member 9 (TNFSF9) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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