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ATF-1 Polyclonal Antibody

ATF-1 Polyclonal Antibody

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Specification

Product name ATF-1 Polyclonal Antibody
Immunogen Synthesized peptide derived from the C-terminal region of human ATF-1 at AA range: 150-230
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative ATF1; Cyclic AMP-dependent transcription factor ATF-1; cAMP-dependent transcription factor ATF-1; Activating transcription factor 1; Protein TREB36

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background ATF1 encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein (activating transcription factor 1) is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. ATF1 has a pseudogene on chromosome 6.
Gene ID 466
Alternative ATF1; Cyclic AMP-dependent transcription factor ATF-1; cAMP-dependent transcription factor ATF-1; Activating transcription factor 1; Protein TREB36
Others ATF-1 Polyclonal Antibody detects endogenous levels of ATF-1 protein.
Accession P18846

Image & description

Fig.1. Western Blot analysis of various cells using ATF-1 Polyclonal Antibody diluted at 1:500.

Fig.1. Western Blot analysis of various cells using ATF-1 Polyclonal Antibody diluted at 1:500.

Fig.2. Western blot analysis of 293T 3T3 HELA KB SH-SY5Y lysis using ATF-1 antibody. Antibody was diluted at 1:500.

Fig.2. Western blot analysis of 293T 3T3 HELA KB SH-SY5Y lysis using ATF-1 antibody. Antibody was diluted at 1:500.

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