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Human Solute carrier family 12 member 1 (SLC12A1) ELISA Kit

Human Solute carrier family 12 member 1 (SLC12A1) ELISA Kit

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Specification

Product name Human Solute carrier family 12 member 1 (SLC12A1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Solute carrier family 12 member 1 (SLC12A1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC12A1 in samples. An antibody specific for SLC12A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC12A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC12A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC12A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SLC12A1; BSC1; MGC48843; NKCC2; NKCC2A variant A; Na-K-2Cl cotransporter; sodium potassium chloride cotransporter 2

Product Properties

Kit components • Human Solute carrier family 12 member 1 microplate
• Human Solute carrier family 12 member 1 standard
• Human Solute carrier family 12 member 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Solute carrier family 12 member 1 (SLC12A1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC12A1. No significant cross-reactivity or interference between Human SLC12A1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The Na-K-Cl cotransporters are a family of integral membrane proteins that mediate the coupled transport of Na+, K+, and Cl- across the plasma membrane.NKCC2 protein is encoded in 26 exons, and the coding region spans 80 kb of genomic DNA with introns that range in length from 120 bp to 15 kb. The location of intron/exon boundaries are very similar to those seen in SLC10A3 through exon 19. Three alternative forms of exon 4 are encoded in genomic DNA, as reported in other species.The gene, symbolized SLC12A1 in human and mouse, is structurally related to another Na-K-Cl cotransporter (SLC12A2; previously called NKCC1) which, unlike the kidney-specific SLC12A1, is expressed in many tissues, including the basolateral membrane of secretory epithelia, where it mediates active chloride secretion.
Gene ID 6557
Alternative SLC12A1; BSC1; MGC48843; NKCC2; NKCC2A variant A; Na-K-2Cl cotransporter; sodium potassium chloride cotransporter 2
Accession Q13621

Image & description

Fig.1. Human Solute carrier family 12 member 1 (SLC12A1) Standard Curve.

Fig.1. Human Solute carrier family 12 member 1 (SLC12A1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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