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Rat Mitochondrial uncoupling protein 2 (UCP2) ELISA Kit

Rat Mitochondrial uncoupling protein 2 (UCP2) ELISA Kit

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Specification

Product name Rat Mitochondrial uncoupling protein 2 (UCP2) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Mitochondrial uncoupling protein 2 (UCP2) ELISA Kit employs a two-site sandwich ELISA to quantitate UCP2 in samples. An antibody specific for UCP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUCP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UCP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UCP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative UCP2; BMIQ4; SLC25A8; UCPH; uncoupling protein 2

Product Properties

Kit components • Rat Mitochondrial uncoupling protein 2 microplate
• Rat Mitochondrial uncoupling protein 2 standard
• Rat Mitochondrial uncoupling protein 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Mitochondrial uncoupling protein 2 (UCP2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat UCP2. No significant cross-reactivity or interference between Rat UCP2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background UCP3 has tissue-specific transcription initiation with other transcription initiation sites upstream of SM-1. Chromosomal order is 5'-UCP3-UCP2-3'. Two splice variants have been found for this gene.Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known.
Gene ID 54315
Alternative UCP2; BMIQ4; SLC25A8; UCPH; uncoupling protein 2
Accession P56500

Image & description

Fig.1. Rat Mitochondrial uncoupling protein 2 (UCP2) Standard Curve.

Fig.1. Rat Mitochondrial uncoupling protein 2 (UCP2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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