|Product name||HRP, Donkey Anti-Sheep IgG|
|Immunogen||Sheep IgG whole molecule|
|Applications||ELISA, IHC-P, WB|
|Applications notes||Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000-1:100000), IHC-P (1:500-1:2000), ELISA (1:5000-1:100000).|
|Preparation method||Affinity purified using solid phase Sheep IgG (H&L) with finally > 95% purity based on SDS-PAGE|
|Storage buffer||Liquid in PBS, pH 7.4, containing 1% BSA as stablizer and 50% Glycerol.|
|Storage instructions||Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.|
|Others||The antibody reacts with whole molecule sheep IgG. It also reacts with heavy chains of sheep IgG, and light chains of all other sheep immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.|
Author：Lin Zhang, Hai-Yun Li, et al Publication name：Frontiers in Immunology IF：4.71
Author：Zhe-Kun Jia, Hai-Yun Li, et al Publication name：Frontiers in Immunology IF：4.71
Author：Han Y, Liu G, Jiang X, et al Publication name：Vaccine IF：3.18
Author：Yan-Guo Han, Gui-Qiong Liu, et al Publication name：Asian-Australasian Journal of Animal Sciences IF：1.58
1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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