| Product name | Na+/K+-ATPase α1 Rabbit Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human Na+/K+-ATPase α1 around the non-phosphorylation site of S16 |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | ELISA, IF, IHC-P, WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Alternative | ATP1A1; Sodium/potassium-transporting ATPase subunit alpha-1; Na(+)/K(+) ATPase alpha-1 subunit; Sodium pump subunit alpha-1 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Storage buffer | PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Sodium/potassium-transporting ATPase subunit alpha-1 encoded by ATP1A1 belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Gene ID | 476 |
| Alternative | ATP1A1; Sodium/potassium-transporting ATPase subunit alpha-1; Na(+)/K(+) ATPase alpha-1 subunit; Sodium pump subunit alpha-1 |
| Others | Na+/K+-ATPase α1 Polyclonal Antibody detects endogenous levels of Na+/K+-ATPase α1 protein. |
| Accession | P05023 |
| Observed Band(KD) | 112 |
Fig.1. Western Blot analysis of various cells using Na+/K+-ATPase α1 Polyclonal Antibody diluted at 1:1000.
Fig.2. Western Blot analysis of 293 cells using Na+/K+-ATPase α1 Polyclonal Antibody diluted at 1:1000.
Author：Jiang R, Wang M, Shen X, Huang S, Han J, Li L, Xu Z, Jiang C, Zhou Q, Feng X, Publication name：Stem Cell Res Ther IF：5.116
Author：F Xiaoli, Z Yaqing, L Ruhui, L Xuan, C Aijie Publication name：Journal of Hazardous IF：4.5
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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