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Human Neuronal-specific septin-3 (SEPT3) ELISA Kit

Human Neuronal-specific septin-3 (SEPT3) ELISA Kit

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Specification

Product name Human Neuronal-specific septin-3 (SEPT3) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Neuronal-specific septin-3 (SEPT3) ELISA Kit employs a two-site sandwich ELISA to quantitate SEPT3 in samples. An antibody specific for SEPT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEPT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEPT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEPT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SEPT3; CTA-250D10.3; MGC133218; SEP3; bK250D10.3; neuronal-specific septin 3

Product Properties

Kit components • Human Neuronal-specific septin-3 microplate
• Human Neuronal-specific septin-3 standard
• Human Neuronal-specific septin-3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Neuronal-specific septin-3 (SEPT3) ELISA Kit has high sensitivity and excellent specificity for detection of Human SEPT3. No significant cross-reactivity or interference between Human SEPT3 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Neuronal-specific septin-3 belongs to the septin family of GTPases. Members of this family are required for cytokinesis. Expression is upregulated by retinoic acid in a human teratocarcinoma cell line. The specific function of this gene has not been determined. Alternative splicing of this gene results in multiple transcript variants. SEP3 shares almost 100% sequence identity with mouse neuronal-specific septin-3 and rat brain-specific G-septin. Alternative splicing leads to 3 SEP3 isoforms, SEP3A, SEP3B, and SEP3C, which encode proteins of 345, 337, and 71 amino acids, respectively. SEP3A and SEP3B contain a set of consensus sequence motifs for GTP binding, 2 predicted phosphorylation sites for PKG, and a conserved binding motif for SH3 domain-containing proteins.
Gene ID 618235
Alternative SEPT3; CTA-250D10.3; MGC133218; SEP3; bK250D10.3; neuronal-specific septin 3
Accession Q08DM7

Image & description

Fig.1. Human Neuronal-specific septin-3 (SEPT3) Standard Curve.

Fig.1. Human Neuronal-specific septin-3 (SEPT3) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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