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Human Histone-lysine N-methyltransferase SETD7 (SETD7) ELISA Kit

Human Histone-lysine N-methyltransferase SETD7 (SETD7) ELISA Kit

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Specification

Product name Human Histone-lysine N-methyltransferase SETD7 (SETD7) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Histone-lysine N-methyltransferase SETD7 (SETD7) ELISA Kit employs a two-site sandwich ELISA to quantitate SETD7 in samples. An antibody specific for SETD7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySETD7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SETD7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SETD7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SETD7; FLJ21193; KIAA1717; KMT7; SET7; SET7/9; SET9; H3-K4-HMTase; SET domain-containing protein 7; histone H3-K4 methyltransferase; histone H3-lysine 4-specific methyltransferase

Product Properties

Kit components • Human Histone-lysine N-methyltransferase SETD7 microplate
• Human Histone-lysine N-methyltransferase SETD7 standard
• Human Histone-lysine N-methyltransferase SETD7 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Histone-lysine N-methyltransferase SETD7 (SETD7) ELISA Kit has high sensitivity and excellent specificity for detection of Human SETD7. No significant cross-reactivity or interference between Human SETD7 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Methylation of histone H3 at lysine-9 (K9) by SUV39H1 and the subsequent recruitment of heterochromatin protein-1 (HP1) is linked to gene silencing. In addition to K9, H3 methylation also occurs at K4, K27, and K36. SET7 methylates H3-K4 in vitro and in vivo and that methylation of H3-K4 and methylation of H3-K9 inhibit each other. Furthermore, H3-K4 methylation and H3-K9 methylation by SET7 and SUV39H1, respectively, were found to have differential effects on subsequent histone acetylation by p300. This study provided a molecular explanation to the differential effects of H3-K4 and H3-K9 methylation on transcription.Set9 regulates the expression of p53 target genes in a manner dependent on the p53 methylation site.
Gene ID 80854
Alternative SETD7; FLJ21193; KIAA1717; KMT7; SET7; SET7/9; SET9; H3-K4-HMTase; SET domain-containing protein 7; histone H3-K4 methyltransferase; histone H3-lysine 4-specific methyltransferase
Accession Q8WTS6

Image & description

Fig.1. Human Histone-lysine N-methyltransferase SETD7 (SETD7) Standard Curve.

Fig.1. Human Histone-lysine N-methyltransferase SETD7 (SETD7) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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