|Product name||Mouse Triosephosphate isomerase (TPI1) ELISA Kit|
|Applications notes||This Mouse Triosephosphate isomerase (TPI1) ELISA Kit employs a two-site sandwich ELISA to quantitate TPI1 in samples. An antibody specific for TPI1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTPI1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPI1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPI1 bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Alternative||TPI1; MGC88108; TPI;|
|Kit components||• Mouse Triosephosphate isomerase microplate
• Mouse Triosephosphate isomerase standard
• Mouse Triosephosphate isomerase detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Mouse Triosephosphate isomerase (TPI1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TPI1. No significant cross-reactivity or interference between Mouse TPI1 and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||TPI1 encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. Spliceosomal introns are present in the nuclear protein-coding genes of most eukaryotic organisms, but they have not been detected in several eukaryotic protist phyla or in eubacteria, archaebacteria, and organelles. Two major theories had emerged in the continuing debate on the origin of these introns.|
|Alternative||TPI1; MGC88108; TPI;|
Fig.1. Mouse Triosephosphate isomerase (TPI1) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.