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Human Secretin (SCT) ELISA Kit

Human Secretin (SCT) ELISA Kit

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Specification

Product name Human Secretin (SCT) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Secretin (SCT) ELISA Kit employs a two-site sandwich ELISA to quantitate SCT in samples. An antibody specific for SCT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySCT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SCT

Product Properties

Kit components • Human Secretin microplate
• Human Secretin standard
• Human Secretin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Secretin (SCT) ELISA Kit has high sensitivity and excellent specificity for detection of Human SCT. No significant cross-reactivity or interference between Human SCT and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Secretin is a hormone that controls the secretions into the duodenum, and also separately, water homeostasis throughout the body. Acting as an anti-Müllerian hormone it is produced in the S cells of the duodenum in the crypts of Lieberkühn. Its effect is to regulate the pH of the duodenal contents via the control of gastric acid secretion and buffering with bicarbonate. It is notable for being the first hormone to be identified. In humans, the secretin peptide is encoded by the SCT gene. It has recently been discovered to play a role in osmoregulation in the hypothalamus, pituitary, and kidney. Secretin is a linear peptide hormone, which is composed of 27 amino acids and has a molecular weight of 3055. A helix is formed in the amino acids between positions 5 and 13.
Gene ID 6343
Alternative SCT
Accession P09683

Image & description

Fig.1. Human Secretin (SCT) Standard Curve.

Fig.1. Human Secretin (SCT) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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